For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.

Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Use for fast and easy preparative purifications either alone or connected in series. HiTrap™ Streptavidin HP Column are convenient and reliable prepacked columns.

Prepacked with Superose™ 4 FF, a highly cross-linked 4% agarose matrix that provides excellent physical stability and chromatographic qualities

Offer good recovery and low nonspecific adsorption. Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules. Enable gel filtration at laboratory and industrial scale, and may be purchased in pre-packed columns.

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Suitable for gel filtration

Used for micro-scale (μg) preparative purification and for characterization and analysis of small volumes (4 to 50μL) of proteins.  Cytiva Lifescience™ Superdex™ 200 Increase 3.2/300 Prepacked Precision Column desigened for size exclusion chromatography.

Used for purification of DNA from small molecules.  Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.

Suitable for gel filtration

Suitable for gel filtration

Choose from a wide range of bead-formed agarose-based gel filtration media. Cytiva Life Sciences™ Sepharose Agarose Gel Filtration Media shows good separation over a wide molecular weight range.

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000.  Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. Cytiva Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Gel filtration medium for desalting and buffer exchange of peptides and small biomolecules

Commonly used for desalting and buffer exchange of very large biomolecules

Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins

High performance gel filtration media for scaleup to preparative purifications of biomolecules

Superdex 30 prep grade (PG) size exclusion chromatography (gel filtration) media prepacked into a HiLoad 26/600 column is optimized for the separation of proteins and peptides, but can also be used with success to separate oligosaccharides.

Maximum recovery and minimum nonspecific adsorption

Remove or purify serine proteases such as thrombin. Cytiva Life Sciences™ HiTrap™ Benzamidine FF (high sub) Columns are prepacked with Benzamidine Sepharose 4 Fast Flow (high sub).

Classic gel filtration medium

Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL

Maximum recovery and minimum nonspecific adsorption

Use for rapid purification of protein A and protein A fusion conjugates. IgG Sepharose™ 6 Fast Flow Medium achieves the highest flowrates.