Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium. STREPTAVIDIN SEPHAROSE HP,5 ML

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories MAGNETIC STAND-96 1 STANDFor the paramagnetic bead precipitation from

Nuclease-free, pyrogen-free water from any MilliporeSigma™ Ultrapure™ water system BIOPAK UF CARTRIDGE

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. GE Healthcare Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification. Prepacked column, Superdex 200 Increase 10/300 GL

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors THROMBIN PROTEASE

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments PROTEASE INH EDTA-FREE SAMPLE 1 VIAL

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay. 30RXN Chromogenic Endotoxin Quant Kit

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. 5 ML HALT(TM) PROTEASE INHIBITOR COCKTAIL, EDTA-FRee 100x 5mL 4C,Ice Pack Store in Fridge at 4C

Thermo Scientific™ Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+] 200ML Sodium Dodecyl Sulfate (SDS) 10% Solution (Electrophoresis, DNase RNase & protease free)

Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents. CELL SURFACE PROT ISOLATION KITStore in Fridge at 4°C

Holds up to 24 microcentrifuge tubes MAGNARACK 24each

1 ML Protino® Ni-NTA agarose sample

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation. 1 ML PIERCE STREPTAVIDIN MAGNETIC BEADS 1ML STOREin Fridge at 4C

Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent. 250 ML PIERCE IP LYSIS BUFFER FOR 25,000CM2 MONOLAyer or 25g cell paste 250mL Store in fridge at 4C

Use Mono Q or S precision columns for fast, sensitive, and high-resolution ion exchange separations of biomolecules at micropreparative scale. GE Healthcare Life Sciences™ Mono Q or S PC, Columns Prepacked with MonoBeads™ achieve maximum resolution from the efficiency of small, perfectly spherical monodispersed particles. MONO Q 10/100 GL

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments LG PROTEASE INH SAMPLE 1 VIAL

Designed for experimental protocols that require volumes exceeding the capacity of conventional shallow well plates 24 DEEP WELL RND BTM BLOCKThe 24 Deep Well RB Block is designed for

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media. X5 HISTrap Fast flow 5mL

Use for cleanup of biomolecules with a molecular weight  >5000 M_r in sample volumes up to 0.5mL.  GE Healthcare Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked. X50 PD MINITRAP G-25

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors. Halt Protease & Phosphatase Inhibitor Cocktail,EDTA free, 10ml

Concentrate and desalt biological samples and perform buffer exchange with disposable polyethersulfone (PES)-membrane ultrafiltration centrifugal devices. X25 Protein concentrator PES 100K MWCO 0.5mL

Designed for method optimization and parameter screening 4.7 ML HISCREEN CAPTO Q 4.7ML STORE AT AMBIENTStore at Room Temperature

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 IUPAC Name: 2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethanol SMILES: CC(C)(C)CC(C)(C)C1=CC=C(C=C1)OCCO 500ML Triton X-100 for Electrophoresis

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates. X5 HisPur Ni-NTA spin column, 1mL resin bed Store

Perform small-scale affinity methods, such as immunoprecipitation assays with agarose beads using these empty spin columns and cups (5 to 500µL). X72 Microcentrifuge tube Pierce Handee 1.5mL

Fast, efficient removal of unincorporated dye from the labeled exosomes X30 EXOSOME SPIN COLUMNS (MW 3000)

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques. X50 Funnel Pierce disposablepolypropylene (pack