Corning

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A Wide Selection for All Your Stem Cell Needs

Corning is committed to helping orchestrate ground breaking discoveries in the Stem Cell field, from fundamental research to cell therapies. As a leader in cell culture, Corning provides advanced cell culture surfaces, vessels, media, and closed systems to ensure cell viability, reproducibility, and regulatory compliance.

Media

Corning® NutriStem® hPSC XF Medium


Support hiPS and hES growth and expansion in a feeder-free environment with NutriStem hPSC XG Medium from Corning.

Enhance the single-cell culture of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells in a defined, xeno- and serum-free environment. Without the need for high levels of bFGF and other stimulatory growth factors or cytokines, low-protein NutriStem medium contains only the most essential components required for successful hiPSC and hESC maintenance, growth, and downstream differentiation.

Complete and ready-to-use, NutriStem provides a simplified medium for consistent performance, as well as increased reproducibility in hPSC long-term culture. 

Superior Growth

NutriStem medium enables excellent proliferation of undifferentiated hES and hiPS cells. Proliferation of H1 hES cells cultured in Corning® Matrigel® Matrix-coated 96-well plates in NutriStem and the leading competing medium for feeder-free culture. Medium was changed and proliferation was assessed every 24 hours in culture.

Normal Colony Morphology

H1 hES cells (panel A) and ACS-1014 hiPS cells (panel B) cultured in Corning NutriStem hPSC XF Medium on Corning Matrigel matrix-coated plates display colony morphologies typical of normal feeder-free hES and hiPS cell cultures, including a uniform colony of tightly compacted cells and distinct colony edges.

Embryoid Body Formation

Embryoid bodies (EBs) were generated from H9.2 hES cells cultured for 16 passages in NutriStem hPSC XF Medium on Matrigel matrix as an evaluation of pluripotency. The pluripotent H9.2 cells were suspended in serum-supplemented medium, where they spontaneously formed EBs containing cells of embryonic germ layers. The following cell types were identified by examination of the histological sections of 14-day-old EBs stained with H&E: (A) neural rosette (ectoderm), (B) neural rosette stained with Tubulin, (C) primitive blood vessels (mesoderm), and (D) megakaryocytes (mesoderm).

Taratoma Formation

H9.2 hES cells were cultured for 11 passages in NutriStem hPSC XF Medium using a human foreskin fibroblast (HFF) feeder layer. The hES cells were subsequently injected into the hind leg muscle of SCID-beige mice for in vitro evaluation of pluripotency. The following tissues from all three germ layers were identified in H&E-stained histological sections of the teratoma 12 weeks post-injection: (A) cartilage (mesoderm), (B) epithelium (endoderm), and (C) neural rosette (ectoderm).

 

Instructions for Use:

Corning NutriStem hPSC XF medium instructions for use for the growth and expansion of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells in a feeder-free environment

 

For more information please refer to the PDF available here

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Extra cellular matrix

Corning® Matrigel® hESC-Qualified Matrix, *LDEV-Free


Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality:

  • Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
  • Extensive PCR testing is performed to screen for a number of pathogens
  • Tested and found negative for bacteria, fungi, and mycoplasma
  • Protein concentrations determined by Lowry method
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay
  • Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
  • Biological activity is determined for each lot using a neurite outgrowth assay.

 

Preparation and Storage:

Store at -20°C. Avoid multiple freeze-thaws.

 

Guidelines for Use:

For more information please refer to the PDF available here

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Cell culture surfaces

Corning® rLaminin-521 (Human)


Support long term self-renewal of human pluroportent stem cells (hPSC) and neureal stem cells (NSCs)

Corning ® rLaminin-521 (Human) is an animal-component-free recombinant heterotrimer composed of a5, B2, and y1 chains expressed in a mammalian cell culture system. The Corning rLaminin-521 (Human) surface supports such key benefits as ROCK inhibitor independent, single-cell expansion of PSCs and inhibition of spontaneous differentiation, improving hPSC culture ease and efficiency.

A collaboration focused on bringing together better solutions for stem cell research

Corning has partnered with BioLamina for the supply of recombinant human laminin 521 (rLaminin-521), a next generation surface that enables feeder-free, single cell passaging of pluripotent stem cells (PSCs) without ROCK-inhibitor. Now we've gone one step further and are working together to combine BioLamina’s expertise in recombinant laminin expression with Corning’s extensive line of vessels and proprietary surface coating technologies. This partnership brings robust and scalable expansion solutions to the emerging stem cell processing market, enabling our customers to scale-up effectively and mitigate risk.

Now backed by Corning’s large network, worldwide distribution channels, and cell culture portfolio, researchers globally have better access and availability to rLaminin-521 (Human) than ever before. 

rLaminin-521 (Human) joins the Corning ECM mimetic and advanced surfaces family: animal component-free surfaces suited for stem and progenitor cell expansion under cGMP processing environments.

Products

Characteristics and Applications
Application A robust, animal component-free substitute enabling ROCK-independent, single-cell passaging of pluripotent stem cells in defined media environments
Surface Technology Passively coated, full length recombinant Laminin protein
Cell types and environment - Human pluripotent stem cells (SF, XF, AF)
-Human embryonic stem cells
- Induced pluripotent stem cells
- Human neural progenitor cells (SF)
Shelf-life 24 months when stored at -20°C
Vialed Formats (Cat.No./Qty.) 354220|20 XXg
354221|100 XXXg
354222|1 mg
354223| 5mg
Options Please inquire about pre-coated vessel options
SF = serum-free media, XF = xeno-free media, AF = animal free media

Features of Corning rLaminin-521 (Human)

  • Animal-component-free recombinant protein
  • Enables ROCK-independent single cell passaging
  • Easy to self-coat
  • Available in 0.1mg aliquots for easy handling
  • Compatible with multiple hPSC culture media and dissociation reagents
  • Supports undifferentiated expansion of hPSC
  • Suitable for defined xeno-free and animal-free culture enviroment

Performance

Effects of Corning rLaminin-521 (Human)

Morphology

hESC cultured on rLaminin-521 (Human) in xeno-free medium exhibited characteristic colony morphology with a high nuclear-to-cytoplasm ratio

Marker Expression

Markers for undifferentiated hPSC were determined by immunocytochemistry. Image analysis indicates Oct-4 (green) expression in the cells. Nuclei were stained with DAPI (blue).

Cells

Cells that Culture Successfully on Corning rLaminin-521 (Human) Surface
Stem and Progenitor Cell Expansion Human embryonic stem cells (hESC)
Human induced pluripotent stem cells (hiPSC)
Neuronal stem cell
In Vitro Differentiation of Pluripotent Stem Cells hESC, hiPSC, mESC (germ cells layers: ectoderm, mesoderm, endoderm; hematopoietic progenitorl definitive differentiation; cardiomyocytes) hESC, hiPSC (neuronal)
Human NPCs (differentiation to neuronal cells)

Cell culture vessels

Falcon Cell Culture Multi-Layer Flasks


Increase productivity by enabling to grow more cells faster and easier. 3- or 5-layer formats provide 525 cm² and 875 cm² cell growth surface area, respectively

  • Even distribution of media across all layers for homogeneous cell growth
  • Ability to mix cells and reagents in the Falcon Multi-Flask saves time and reduces risk of contamination
  • Flexible design allows to pour or aspirate/recover cells using a pipet
  • Consistent surface treatment for predictable scale-up
  • Lot number printed on each flask for traceability
  • Manufactured in compliance to cGMP standards

For more information please refer to the PDF available here - KEEP THIS ONE??

PureCoat ECM Mimetic Cultureware

Create a fully defined cell culture environment-serum-, xeno-, or animal-free

Support the growth of stem and primary cells across a broad range of defined media formulations with the choice of two ECM mimetic types:

PureCoat ECM mimetic fibronectin peptide consists of RGD sequences to support the attachment of cell types that require fibronectic coating, such as alpha-5 integrin-postiive cells.

PureCoat ECM mimetic collagin I peptide supports the attachment of collagen I-dependent cell types, including alpha 2 integrin-positive cells, among others

For more information please refer to the PDF available here (Attached)

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Resources Products

Corning™ Reusable PFA Low-Form Beakers

X12 Reusable Plastic Low Form Beaker, PFA, 100ml,Nonsterile

Corning™ 1536-Well Nontreated Polystyrene Plates

Superior flatness minimizes variability within a plate and between plates X50 Microplate, 1536 Well, PS, Black, Flat Bottom, Not Treated, no Lid, Nonsterile, Bulk

Corning™ LSE™ Orbital Shaker Clamps

For holding Erlenmeyer flasks clamp for 250ml erlenmyer flask

Corning™ Universal Fit Pipet Tips: Racked

Designed to assure a reliable fit with major brand pipetters X960 tip 1-200µL, Universal Fit, PP, Natural, Nonsterile, 96/rack

Corning™ ProCulture Media Handling Fittings

Introduce medium aseptically into larger Spinner Flasks with vertical sidearms during cell culture Sidearm Fitting, Dual, Media Handling, 8 L, 15 L,1/8, 1/4 Tubing

Corning™ LSE™ Fixed-Angle Rotors

Rotors with different tube size and capacity configurations rotor, 6x50ml fixed angle rotor

Corning™ 96-Well Special Optics Low Fluorescence Assay Plates

Ideal for spectroscopy, polarization, and imaging X100 96-Well plate, Special Optics, PS, Black/Clear Flat Bottom, TC-Treated, no Lid, Sterile, Bulk

Corning™ Costar™ Disposable Aspirating Pipets

Constructed of polystyrene for strength, clarity, and durability. Corning™ Costar™ Disposable Aspirating Pipets are ideal for aseptically aspirating liquids by using vacuum suction. X1000 Aspirating Pipet, PS, 1ml, No Graduations, Bulk Packed, Sterile, 50/Bag

Corning™ DeckWorks™ Bulk Pipet Tips

Compatible with DeckWorks racks and packaged in resealable polyethylene bags X1000 DeckWorks tip, 1-200µL, PP, Graduated, Natural, Nonsterile

PYREX™ Disposable Round-Bottom Rimless Glass Tubes

Manufactured from borosilicate glass to reduce pH changes and contaminants. Corning™ PYREX™ Disposable Round-Bottom Rimless Glass Tubes are designed for both tissue culture and general bacteriological work. X1000 Disposable Rimless Culture tube, PYREX, 15x85mm, Bulk Pack, Nonsterile, 250/bag

Corning™ 96-Well Cell Culture Multiwell Plates

Designed for use in most thermal cyclers and offer a rigidity that makes them ideal for high-throughput (HT) automation. X100 96-Well plate Stripwell, 8 Well Strips, PS, Clear, Flat Btm, High Binding, no Lid, Nonsterile

Corning™ Transwell™ Multiple Well Plate with Permeable Polyester Membrane Inserts

Featuring a microscopically transparent polyester membrane that is TC-treated for optimal cell attachment and growth. Corning™ Transwell™ Multiwell Plate with Polyester Membrane Inserts provide better visibility under phase contrast microscopy and allow assessment of cell viability and monolayer formation. X48 Transwell-24 insert, 6.5mm, TC Treated PET Membrane 0.4µm, 12 in 24-well plate w/lid, Sterile

Corning™ Sealing Mat for 384-Well Polypropylene Storage Plates

Ideal for use in long-term, low temperature compound storage X50 Sealing Mat, for 384 Well DeepWell, PP, Chemical Resistant, Nonsterile, Bulk

Corning™ Polystyrene Stripette

X200 Stripette, 25mL, Poly Wrap, Polystyrene, w/oTriple Bagged, Sterile

Corning™ DeckWorks™ Low-Binding Hinged Rack Pipet Tips

Designed for quantitative assays in which sample binding can significantly alter results X960 DeckWorks Low Binding tip, 0.1-10µL XL, PP, Graduated, Natural, Nonsterile, 96/hinged rack

Corning™ Costar™ Snap Cap Microcentrifuge Tubes

Features a snap cap with a positive seal that allows for repeated opening and closing with flat-cap surfaces for convenient labeling. Corning™ Costar™ Snap Cap Microcentrifuge Tubes are compatible with leading microcentrifuge rotors. X500 Microcentrifuge tubes 1.7ml, PP, natural, snap cap, non sterile, bulk

Corning™ HTS Transwell™ 24-Well Permeable Support System

Run 24 automated, high-throughput drug transport studies at once. HTS Costar™ HTS Transwell™ 24-Well Permeable Support System is a microplate support system for an array of 24 wells with membrane inserts connected by a rigid, robotics-friendly tray that enables all 24 inserts to be handled as a single unit. X2 HTS Transwell-24 Support, TC treated PC Mbne 3.0µm, in receiver w/24-well plate & lid, Sterile

Corning™ 96-Well Next Generation CrystalEX Protein Crystallization Microplates

Optimized for high-throughput sitting-drop protein crystallization X50 96-Well Crystallization Plate, COC, 3:1, 2µL Conical Flat Bottom, Nonsterile, 10/bag

Corning™ Costar™ Transwell™-COL Collagen-Treated Coated Membrane Insert

Transparent, collagen-treated microporous membranes promote cell attachment and spreading X24 Transwell-12 insert, 12mm, COL-PTFE Mbne 0.4µm, Ind. Wrapped, w/2 12-well plates & lid, Sterile

Corning™ 384-Well Clear Bottom Black or White Polystyrene Microplates

Manufactured from medical grade polystyrene for strength. Corning™ 384-Well Clear Bottom Black or White Polystyrene Microplates permit top or bottom analysis using detection microplate readers. X100 384 square Well plate, PS, Black/Clear Flat Bottom, TC-Treated, with Lid, Sterile, Bulk

Corning™ Microvolume Pipet Tips

Provide accurate, reliable performance in the stated volume range X960 tip 0.5-10µL, fit Eppendorf, PP, Natural, Nonsterile, 96/Rack

Corning™ UV-Transparent Microplates

Designed with a UV-transparent well bottom for determining protein and nucleic acid concentrations. Corning™ UV-Transparent Microplates allows UV absorbance readings with low background, especially at 260 to 280nm. X25 Plate Corning Life Sciences UV contains inner

Corning™ Polystyrene Disposable Culture Tubes

Manufactured from optically clear polystyrene for optimum clarity. Corning™ Polystyrene Disposable Culture Tubes are provided with threaded plug seal caps to prevent leakage. X500 Threaded Culture Tube, PS, 16x125mm, Round Bottom, TC treated, Plug Seal, Sterile, 50/bag

Corning™ CrystalEX 96-Well Protein Crystallization Microplates

Designed for sitting-drop vapor diffusion high throughput protein crystallization X50 96-Well CrystalEX plate, Advanced Polymer, Round Bottom, Nonsterile, 10/bag

Corning™ Closed Systems Erlenmeyer Flasks

X3 Erlenmeyer Flask, 1l, plain, w/aseptic conn, mp

Corning™ Costar™ Sterile Disposable Reagent Reservoirs

Manufactured from modified polystyrene for strength. Corning™ Costar™ Sterile Disposable Reagent Reservoirs are ideal for repetitive filling of multichannel pipetters. X100 Reagent reservoir 50ml, PS, white, Sterile, individually packaged

Corning™ LSE™ Orbital Shaker Platform

Platform includes rubber mat flat platform with rubber mat, for use with orbital shaker

Corning™ 384-Well Clear Polystyrene Microplates

Manufactured from clear medical grade polystyrene for strength and visibility. Corning™ 364-Well Clear Polystyrene Microplates present an excellent solution for cell culture and colorimetric (absorbance) assays. Use 364-well microplates for laboratory miniaturization and automation. X100 384 square Well plate, PS, Clear, Flat Bottom, Not Treated, no Lid, Nonsterile, Bar Code, bulk

Corning™ Ultralow Attachment Flasks

Features a covalently bound hydrogel layer that minimizes cell attachment, protein absorption and cellular activation. Corning™ Ultralow Attachment Flasks have an ergonomic shape that reduces the number of corners, improves cell scraping and allows the use of a larger pipet. X24 Ultra-Low Attachment Rectangular Canted Neck Flask, 75cm², Vent Cap, Sterile, 4/bag

Corning™ Polyethylene Lids for Graduated Disposable Polypropylene Beakers

These replacement snap-on polyethylene lids fit on the Corning 250mL containers 02-540-11. X500 Replacement Snap-On HDPE Lid for 250ml Container, Sterile, 20/bag

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