Corning

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A Wide Selection for All Your Stem Cell Needs

Corning is committed to helping orchestrate ground breaking discoveries in the Stem Cell field, from fundamental research to cell therapies. As a leader in cell culture, Corning provides advanced cell culture surfaces, vessels, media, and closed systems to ensure cell viability, reproducibility, and regulatory compliance.

Media

Corning® NutriStem® hPSC XF Medium


Support hiPS and hES growth and expansion in a feeder-free environment with NutriStem hPSC XG Medium from Corning.

Enhance the single-cell culture of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells in a defined, xeno- and serum-free environment. Without the need for high levels of bFGF and other stimulatory growth factors or cytokines, low-protein NutriStem medium contains only the most essential components required for successful hiPSC and hESC maintenance, growth, and downstream differentiation.

Complete and ready-to-use, NutriStem provides a simplified medium for consistent performance, as well as increased reproducibility in hPSC long-term culture. 

Superior Growth

NutriStem medium enables excellent proliferation of undifferentiated hES and hiPS cells. Proliferation of H1 hES cells cultured in Corning® Matrigel® Matrix-coated 96-well plates in NutriStem and the leading competing medium for feeder-free culture. Medium was changed and proliferation was assessed every 24 hours in culture.

Vertical Gel Electrophoresis

H1 hES cells (panel A) and ACS-1014 hiPS cells (panel B) cultured in Corning NutriStem hPSC XF Medium on Corning Matrigel matrix-coated plates display colony morphologies typical of normal feeder-free hES and hiPS cell cultures, including a uniform colony of tightly compacted cells and distinct colony edges.

Embryoid Body Formation

Embryoid bodies (EBs) were generated from H9.2 hES cells cultured for 16 passages in NutriStem hPSC XF Medium on Matrigel matrix as an evaluation of pluripotency. The pluripotent H9.2 cells were suspended in serum-supplemented medium, where they spontaneously formed EBs containing cells of embryonic germ layers. The following cell types were identified by examination of the histological sections of 14-day-old EBs stained with H&E: (A) neural rosette (ectoderm), (B) neural rosette stained with Tubulin, (C) primitive blood vessels (mesoderm), and (D) megakaryocytes (mesoderm).

Taratoma Formation

H9.2 hES cells were cultured for 11 passages in NutriStem hPSC XF Medium using a human foreskin fibroblast (HFF) feeder layer. The hES cells were subsequently injected into the hind leg muscle of SCID-beige mice for in vitro evaluation of pluripotency. The following tissues from all three germ layers were identified in H&E-stained histological sections of the teratoma 12 weeks post-injection: (A) cartilage (mesoderm), (B) epithelium (endoderm), and (C) neural rosette (ectoderm).

Instructions for Use:

Corning NutriStem hPSC XF medium instructions for use for the growth and expansion of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells in a feeder-free environment

Corning link for more information »

For more information please refer to the PDF available here

CV Product image template
Extra cellular matrix

Corning® Matrigel® hESC-Qualified Matrix, *LDEV-Free


Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality:

  • Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
  • Extensive PCR testing is performed to screen for a number of pathogens
  • Tested and found negative for bacteria, fungi, and mycoplasma
  • Protein concentrations determined by Lowry method
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay
  • Matrigel Matrix gel stability is tested for a period of 14 days at 37°C
  • Biological activity is determined for each lot using a neurite outgrowth assay.

Preparation and Storage:

Store at -20°C. Avoid multiple freeze-thaws.

Guidelines for Use:

For more information please refer to the PDF available here

Corning link for more information »

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Cell culture surfaces

Corning® Laminin Surface


Enable the study of specific mechanisms that dictate cell differentiation and functionality with Corning Laminin surfaces.

Use Corning Laminin surfaces as a thin coating on tissue culture surfaces or as a soluble additive to culture medium to promote cell adhesion, migration, chemotaxis, growth, and differentiation. Laminin/Entactin Complex High Concentration (HC) is a special formulation that Corning has developed to enhance three-dimensional (3D) cell culture applications. Culturing cells in or on gels such as Laminin/Entactin Complex enable the study of specific mechanisms that dictate cell differentiation and functionality.

Applications include:

  • Promotion of neurite outgrowth
  • Reduction of fibroblast migration in neuronal cell cultures
  • Neural crest cell attachment
  • Gene expression in rat cortical neurons
  • Maintenance of undifferentiated pluripotent stem cells

Corning® rLaminin-521

Corning rLaminin-521 (Human) is a heterotrimer composed of α5, β2, and γ1 chains expressed in a mammalian cell culture system. rLaminin-521 (Human) supports long-term self-renewal of human pluripotent stem cells (hPSC), including embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) in defined and xeno-free environments. rLaminin-521 provides additional benefits, including ROCK inhibitor independent single cell expansion of PSCs, which improves hPSC culture ease and efficiency.

Features:

  • Recombinant protein
  • Easy to self-coat
  • Available in 0.1 mg aliquots for easy handling
  • Compatible with multiple hPSC culture media and dissociation reagents
  • Supports undifferentiated expansion of hPSC
  • Suitable for xeno-free, defined culture environment

Corning® rLaminin-521 (human) Frequently Asked Questions

Have a query? Contact us

Laminin-521--35422
Cell culture vessels

Falcon Cell Culture Multi-Layer Flasks


Increase productivity by enabling to grow more cells faster and easier. 3- or 5-layer formats provide 525 cm² and 875 cm² cell growth surface area, respectively

  • Even distribution of media across all layers for homogeneous cell growth
  • Ability to mix cells and reagents in the Falcon Multi-Flask saves time and reduces risk of contamination
  • Flexible design allows to pour or aspirate/recover cells using a pipet
  • Consistent surface treatment for predictable scale-up
  • Lot number printed on each flask for traceability
  • Manufactured in compliance to cGMP standards

For more information please refer to the PDF available here

Corning_Falcon_Cell_Culture_Multi_Flasks
Offers
Resources
Products

Corning™ 1536-Well Nontreated Polystyrene Plates

Superior flatness minimizes variability within a plate and between plates X50 Microplate, 1536 Well, PS, Black, Flat Bottom, Not Treated, no Lid, Nonsterile, Bulk

Corning™ LSE™ Fixed-Angle Rotors

Rotors with different tube size and capacity configurations rotor, 6x50ml fixed angle rotor

Corning™ Universal Fit Pipet Tips: Racked

Designed to assure a reliable fit with major brand pipetters X960 tip 1-200µL, Universal Fit, PP, Natural, Nonsterile, 96/rack

Corning™ ProCulture Media Handling Fittings

Introduce medium aseptically into larger Spinner Flasks with vertical sidearms during cell culture Sidearm Fitting, Dual, Media Handling, 8 L, 15 L,1/8, 1/4 Tubing

Corning™ 96-Well Special Optics Low Fluorescence Assay Plates

Ideal for spectroscopy, polarization, and imaging X100 96-Well plate, Special Optics, PS, Black/Clear Flat Bottom, TC-Treated, no Lid, Sterile, Bulk

Corning™ 384-Well Spheroid Microplate

Treated with an ultra-low attachment surface to enable uniform and reproducible 3D multicellular spheroid formation. Corning™ 384-Well Spheroid Microplate permit direct microscopic viewing and readings for top and bottom reading instruments. X50 Spheroid Microplate, 384 well, Black with Clear Round bottom, with Lid, ULA, Sterile

Corning™ Costar™ Disposable Aspirating Pipets

Constructed of polystyrene for strength, clarity, and durability. Corning™ Costar™ Disposable Aspirating Pipets are ideal for aseptically aspirating liquids by using vacuum suction. X1000 Aspirating Pipet, PS, 1ml, No Graduations, Bulk Packed, Sterile, 50/Bag

PYREX™ Disposable Round-Bottom Rimless Glass Tubes

Manufactured from borosilicate glass to reduce pH changes and contaminants. Corning™ PYREX™ Disposable Round-Bottom Rimless Glass Tubes are designed for both tissue culture and general bacteriological work. X1000 Disposable Rimless Culture tube, PYREX, 15x85mm, Bulk Pack, Nonsterile, 250/bag

Corning™ 96-Well Cell Culture Multiwell Plates

Designed for use in most thermal cyclers and offer a rigidity that makes them ideal for high-throughput (HT) automation. X100 96-Well plate Stripwell, 8 Well Strips, PS, Clear, Flat Btm, High Binding, no Lid, Nonsterile

Corning™ Transwell™ Multiple Well Plate with Permeable Polyester Membrane Inserts

Featuring a microscopically transparent polyester membrane that is TC-treated for optimal cell attachment and growth. Corning™ Transwell™ Multiwell Plate with Polyester Membrane Inserts provide better visibility under phase contrast microscopy and allow assessment of cell viability and monolayer formation. X48 Transwell-24 insert, 6.5mm, TC Treated PET Membrane 0.4µm, 12 in 24-well plate w/lid, Sterile

Corning™ Sealing Mat for 384-Well Polypropylene Storage Plates

Ideal for use in long-term, low temperature compound storage X50 Sealing Mat, for 384 Well DeepWell, PP, Chemical Resistant, Nonsterile, Bulk

Corning™ LSE™ Orbital Shaker Platform

Platform includes rubber mat flat platform with rubber mat, for use with orbital shaker

Corning™ Polystyrene Stripette

X200 Stripette, 25mL, Poly Wrap, Polystyrene, w/oTriple Bagged, Sterile

PYREX™ Media Bottle High-Temperature Caps

With drip-free pouring ring X10 GL45 Red Open Top High Temperature (180°C) PBT Screw Cap, Nonsterile, Bulk

Corning™ DeckWorks™ Reload Systems with Standard Tips

Packaged in transparent trays for easy tip identification. Corning™ Deckworks™ Reload Systems with Standard Tips provide convenient and economical reloading of durable hinged racks. X3840 DeckWorks tip, 1-200µL, PP, Graduated, Natural, Nonsterile, 96/reload deck

Corning™ Costar™ Snap Cap Microcentrifuge Tubes

Features a snap cap with a positive seal that allows for repeated opening and closing with flat-cap surfaces for convenient labeling. Corning™ Costar™ Snap Cap Microcentrifuge Tubes are compatible with leading microcentrifuge rotors. X500 Microcentrifuge tubes 1.7ml, PP, natural, snap cap, non sterile, bulk

Corning™ HTS Transwell™ 24-Well Permeable Support System

Run 24 automated, high-throughput drug transport studies at once. HTS Costar™ HTS Transwell™ 24-Well Permeable Support System is a microplate support system for an array of 24 wells with membrane inserts connected by a rigid, robotics-friendly tray that enables all 24 inserts to be handled as a single unit. X2 HTS Transwell-24 Support, TC treated PC Mbne 3.0µm, in receiver w/24-well plate & lid, Sterile

Corning™ 96-Well Next Generation CrystalEX Protein Crystallization Microplates

Optimized for high-throughput sitting-drop protein crystallization X50 96-Well Crystallization Plate, COC, 3:1, 2µL Conical Flat Bottom, Nonsterile, 10/bag

Corning™ Costar™ Transwell™-COL Collagen-Treated Coated Membrane Insert

Transparent, collagen-treated microporous membranes promote cell attachment and spreading X24 Transwell-12 insert, 12mm, COL-PTFE Mbne 0.4µm, Ind. Wrapped, w/2 12-well plates & lid, Sterile

Corning™ Corning PFA Graduated Low Form Griffin Beaker

X12 Reusable Plastic Low Form Beaker, PFA, 25ml, Nonsterile

Corning™ 384-Well Clear Bottom Black or White Polystyrene Microplates

Manufactured from medical grade polystyrene for strength. Corning™ 384-Well Clear Bottom Black or White Polystyrene Microplates permit top or bottom analysis using detection microplate readers. X100 384 square Well plate, PS, Black/Clear Flat Bottom, TC-Treated, with Lid, Sterile, Bulk

Corning™ 384 Well Flat Clear Bottom Polystyrene TC-Treated Microplates, low flange, with lid, sterile, with generic barcodes

Can be used for both top and bottom reading instruments. Corning™ 384 Well Flat Clear Bottom Polystyrene TC-Treated Microplates have black wall plates for low background fluorescence, minimal light scatter, and reduced crosstalk. X100 Microplate,384 Well,Polystyrene,Black with Clear Flat Bottom,TC-Treated,Sterile,low flange

Corning™ Microvolume Pipet Tips

Provide accurate, reliable performance in the stated volume range X960 tip 0.5-10µL, fit Eppendorf, PP, Natural, Nonsterile, 96/Rack

Corning™ UV-Transparent Microplates

Designed with a UV-transparent well bottom for determining protein and nucleic acid concentrations. Corning™ UV-Transparent Microplates allows UV absorbance readings with low background, especially at 260 to 280nm. X25 Plate Corning Life Sciences UV contains inner

Corning™ Storage Mat Applicator

Quickly applies Storage Mat III to 96-well plates Applicator, for 96 Well Storage Mat, Black

Corning™ Polystyrene Disposable Culture Tubes

Manufactured from optically clear polystyrene for optimum clarity. Corning™ Polystyrene Disposable Culture Tubes are provided with threaded plug seal caps to prevent leakage. X500 Threaded Culture Tube, PS, 16x125mm, Round Bottom, TC treated, Plug Seal, Sterile, 50/bag

Corning™ CrystalEX 96-Well Protein Crystallization Microplates

Designed for sitting-drop vapor diffusion high throughput protein crystallization X50 96-Well CrystalEX plate, Advanced Polymer, Round Bottom, Nonsterile, 10/bag

Corning™ LSE Digital Dry Bath Heater Blocks

dual block, 384 well PCR plate

Corning™ Costar™ Sterile Disposable Reagent Reservoirs

Manufactured from modified polystyrene for strength. Corning™ Costar™ Sterile Disposable Reagent Reservoirs are ideal for repetitive filling of multichannel pipetters. X100 Reagent reservoir 50ml, PS, white, Sterile, individually packaged

Corning™ Fiberglass Prefilters

X100 Fiberglass Prefilters, 49.5mm Square, Nonsterile, , Bulk

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OLD CONTENT
Products

Corning™ 1536-Well Nontreated Polystyrene Plates

Superior flatness minimizes variability within a plate and between plates X50 Microplate, 1536 Well, PS, Black, Flat Bottom, Not Treated, no Lid, Nonsterile, Bulk

Corning™ LSE™ Fixed-Angle Rotors

Rotors with different tube size and capacity configurations rotor, 6x50ml fixed angle rotor

Corning™ Universal Fit Pipet Tips: Racked

Designed to assure a reliable fit with major brand pipetters X960 tip 1-200µL, Universal Fit, PP, Natural, Nonsterile, 96/rack

Corning™ ProCulture Media Handling Fittings

Introduce medium aseptically into larger Spinner Flasks with vertical sidearms during cell culture Sidearm Fitting, Dual, Media Handling, 8 L, 15 L,1/8, 1/4 Tubing

Corning™ 96-Well Special Optics Low Fluorescence Assay Plates

Ideal for spectroscopy, polarization, and imaging X100 96-Well plate, Special Optics, PS, Black/Clear Flat Bottom, TC-Treated, no Lid, Sterile, Bulk

Corning™ 384-Well Spheroid Microplate

Treated with an ultra-low attachment surface to enable uniform and reproducible 3D multicellular spheroid formation. Corning™ 384-Well Spheroid Microplate permit direct microscopic viewing and readings for top and bottom reading instruments. X50 Spheroid Microplate, 384 well, Black with Clear Round bottom, with Lid, ULA, Sterile

Corning™ Costar™ Disposable Aspirating Pipets

Constructed of polystyrene for strength, clarity, and durability. Corning™ Costar™ Disposable Aspirating Pipets are ideal for aseptically aspirating liquids by using vacuum suction. X1000 Aspirating Pipet, PS, 1ml, No Graduations, Bulk Packed, Sterile, 50/Bag

PYREX™ Disposable Round-Bottom Rimless Glass Tubes

Manufactured from borosilicate glass to reduce pH changes and contaminants. Corning™ PYREX™ Disposable Round-Bottom Rimless Glass Tubes are designed for both tissue culture and general bacteriological work. X1000 Disposable Rimless Culture tube, PYREX, 15x85mm, Bulk Pack, Nonsterile, 250/bag

Corning™ 96-Well Cell Culture Multiwell Plates

Designed for use in most thermal cyclers and offer a rigidity that makes them ideal for high-throughput (HT) automation. X100 96-Well plate Stripwell, 8 Well Strips, PS, Clear, Flat Btm, High Binding, no Lid, Nonsterile

Corning™ Transwell™ Multiple Well Plate with Permeable Polyester Membrane Inserts

Featuring a microscopically transparent polyester membrane that is TC-treated for optimal cell attachment and growth. Corning™ Transwell™ Multiwell Plate with Polyester Membrane Inserts provide better visibility under phase contrast microscopy and allow assessment of cell viability and monolayer formation. X48 Transwell-24 insert, 6.5mm, TC Treated PET Membrane 0.4µm, 12 in 24-well plate w/lid, Sterile

Corning™ Sealing Mat for 384-Well Polypropylene Storage Plates

Ideal for use in long-term, low temperature compound storage X50 Sealing Mat, for 384 Well DeepWell, PP, Chemical Resistant, Nonsterile, Bulk

Corning™ LSE™ Orbital Shaker Platform

Platform includes rubber mat flat platform with rubber mat, for use with orbital shaker

Corning™ Polystyrene Stripette

X200 Stripette, 25mL, Poly Wrap, Polystyrene, w/oTriple Bagged, Sterile

PYREX™ Media Bottle High-Temperature Caps

With drip-free pouring ring X10 GL45 Red Open Top High Temperature (180°C) PBT Screw Cap, Nonsterile, Bulk

Corning™ DeckWorks™ Reload Systems with Standard Tips

Packaged in transparent trays for easy tip identification. Corning™ Deckworks™ Reload Systems with Standard Tips provide convenient and economical reloading of durable hinged racks. X3840 DeckWorks tip, 1-200µL, PP, Graduated, Natural, Nonsterile, 96/reload deck

Corning™ Costar™ Snap Cap Microcentrifuge Tubes

Features a snap cap with a positive seal that allows for repeated opening and closing with flat-cap surfaces for convenient labeling. Corning™ Costar™ Snap Cap Microcentrifuge Tubes are compatible with leading microcentrifuge rotors. X500 Microcentrifuge tubes 1.7ml, PP, natural, snap cap, non sterile, bulk

Corning™ HTS Transwell™ 24-Well Permeable Support System

Run 24 automated, high-throughput drug transport studies at once. HTS Costar™ HTS Transwell™ 24-Well Permeable Support System is a microplate support system for an array of 24 wells with membrane inserts connected by a rigid, robotics-friendly tray that enables all 24 inserts to be handled as a single unit. X2 HTS Transwell-24 Support, TC treated PC Mbne 3.0µm, in receiver w/24-well plate & lid, Sterile

Corning™ 96-Well Next Generation CrystalEX Protein Crystallization Microplates

Optimized for high-throughput sitting-drop protein crystallization X50 96-Well Crystallization Plate, COC, 3:1, 2µL Conical Flat Bottom, Nonsterile, 10/bag

Corning™ Costar™ Transwell™-COL Collagen-Treated Coated Membrane Insert

Transparent, collagen-treated microporous membranes promote cell attachment and spreading X24 Transwell-12 insert, 12mm, COL-PTFE Mbne 0.4µm, Ind. Wrapped, w/2 12-well plates & lid, Sterile

Corning™ Corning PFA Graduated Low Form Griffin Beaker

X12 Reusable Plastic Low Form Beaker, PFA, 25ml, Nonsterile

Corning™ 384-Well Clear Bottom Black or White Polystyrene Microplates

Manufactured from medical grade polystyrene for strength. Corning™ 384-Well Clear Bottom Black or White Polystyrene Microplates permit top or bottom analysis using detection microplate readers. X100 384 square Well plate, PS, Black/Clear Flat Bottom, TC-Treated, with Lid, Sterile, Bulk

Corning™ 384 Well Flat Clear Bottom Polystyrene TC-Treated Microplates, low flange, with lid, sterile, with generic barcodes

Can be used for both top and bottom reading instruments. Corning™ 384 Well Flat Clear Bottom Polystyrene TC-Treated Microplates have black wall plates for low background fluorescence, minimal light scatter, and reduced crosstalk. X100 Microplate,384 Well,Polystyrene,Black with Clear Flat Bottom,TC-Treated,Sterile,low flange

Corning™ Microvolume Pipet Tips

Provide accurate, reliable performance in the stated volume range X960 tip 0.5-10µL, fit Eppendorf, PP, Natural, Nonsterile, 96/Rack

Corning™ UV-Transparent Microplates

Designed with a UV-transparent well bottom for determining protein and nucleic acid concentrations. Corning™ UV-Transparent Microplates allows UV absorbance readings with low background, especially at 260 to 280nm. X25 Plate Corning Life Sciences UV contains inner

Corning™ Storage Mat Applicator

Quickly applies Storage Mat III to 96-well plates Applicator, for 96 Well Storage Mat, Black

Corning™ Polystyrene Disposable Culture Tubes

Manufactured from optically clear polystyrene for optimum clarity. Corning™ Polystyrene Disposable Culture Tubes are provided with threaded plug seal caps to prevent leakage. X500 Threaded Culture Tube, PS, 16x125mm, Round Bottom, TC treated, Plug Seal, Sterile, 50/bag

Corning™ CrystalEX 96-Well Protein Crystallization Microplates

Designed for sitting-drop vapor diffusion high throughput protein crystallization X50 96-Well CrystalEX plate, Advanced Polymer, Round Bottom, Nonsterile, 10/bag

Corning™ LSE Digital Dry Bath Heater Blocks

dual block, 384 well PCR plate

Corning™ Costar™ Sterile Disposable Reagent Reservoirs

Manufactured from modified polystyrene for strength. Corning™ Costar™ Sterile Disposable Reagent Reservoirs are ideal for repetitive filling of multichannel pipetters. X100 Reagent reservoir 50ml, PS, white, Sterile, individually packaged

Corning™ Fiberglass Prefilters

X100 Fiberglass Prefilters, 49.5mm Square, Nonsterile, , Bulk

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Extra cellular matrix Media

As Corning Life Sciences premier distributor, we firmly believe that both companies are united in a common goal.

We are both fully committed to meeting customers' unique and challenging needs, providing high quality products and a service that they can rely on.

Take a look at what we can offer when you buy Corning products from Fisher Scientific:

  • Timely deliveries
  • Responsive and friendly customer service
  • Relevant communication in a mode to suit you
  • Unchanged part numbers
  • Field based specialist support
  • Clear pricing via the web
  • Real time stock availability
  • Efficient web-based ordering and product selection

Corning have a 150-year tradition of innovation and develop original solutions for today's emerging life sciences technologies.

Their dedication to quality, technology and innovation has enabled them to produce the world's most comprehensive line of laboratory tools for life science research.

Key product areas are:

  • Assay and HTS
  • A range of microplates in a variety of designs, sizes, materials and surface treatments.
  • Cell Culture
  • Includes dishes, flasks, microplates, Transwell® permeable supports, cryogenic vials,roller bottles, spinner flasks and cellstacks.
  • Liquid Handling
  • The range of products include serological pipettes, centrifuge tubes, graduated cylinders, and Erlenmeyer flasks.
  • Laboratory Filtration
  • The filtration products include syringe filters and vacuum filter systems, as well as storage bottles.
Cell culture vessels
Offers
Resources
Cell culture surfaces