Cell Culture Media
Gibco™ StemPro™-34 SFM (1X)
StemPro™-34 SFM (1X)
Gibco™ Expi293™ Expression Medium
Expi293™ Expression Medium
Gibco™ Cell Dissociation Buffer, enzyme-free, PBS
Animal origin-free
Gibco™ Ham's F-12 Nutrient Mix
Designed for serum-free, single-cell plating of Chinese hamster ovary (CHO) cells
Gibco™ Versene Solution
An EDTA solution for use as a gentle non-enzymatic cell dissociation reagent
Gibco™ AmnioMAX™-II Complete Medium
Fully-supplemented medium developed for short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures
Gibco™ Advanced DMEM/F-12
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ Advanced DMEMF/12 is used in cell culture processing applications.
Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ SFM4Insect™ Media
Support growth of many key insect cell lines and production of a variety of recombinant proteins. HyClone™ SFM4Insect™ Media is free of animal-derived components (ADCF).
Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ DMEM/F12 1:1 Media
Used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. HyClone™ DMEM/F12 1:1 Media supports cell culture growth and biomanufacturing processes and is manufactured using ISO 9001:2000-certified processes.
Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ McCoy's 5A Media
Support cell culture growth and biomanufacturing processes with HyClone™ McCoy's 5A Media, manufactured using ISO 9001-certified processes.
Gibco™ DMEM, high glucose, HEPES, no phenol red
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, HEPES, No Phenol Red is used in cell culture processing applications.
Lonza™ BioWhittaker Insect-XPRESS Insect Cell Culture Media (1X) with L-Glutamine
Designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21). BioWhittaker™ Insect-XPRESS™ Insect Cell Culture Media (1X) with L-Glutamine supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS).
Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, GlutaMAX™ Supplement, Pyruvate is used in human ex vivo tissue and cell culture processing applications.
Gibco™ Opti-MEM™ I Reduced Serum Medium, no phenol red
Improved Minimal Essential Medium that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in cell growth rate or morphology
Gibco™ Ham's F-12K (Kaighn's) Medium
A modification of Ham's F-12 Nutrient Mixture
Gibco™ Glucose Solution
Contains D-glucose at concentration of 200 g/L and is used as serum-free medium supplement suitable for cell culture
Gibco™ DMEM/F-12, GlutaMAX™ supplement
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEMF/12, GlutaMAX™ Supplement is used in cell culture processing applications.
Gibco™ RPMI, no methionine
Developed to culture human leukemic cells in suspension and as monolayer
Gibco™ DMEM/F-12, no phenol red
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEMF/12, No Phenol Red is used in cell culture processing applications.
Gibco™ Essential 8™ Medium
Essential 8™ Medium
Gibco™ IMDM
Iscove's Modified Dulbecco's Medium is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
Gibco™ RPMI 1640 Medium, no folic acid
Originally developed to culture human leukemic cells in suspension and as a monolayer
Gibco™ Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
Gibco™ Neurobasal™-A Medium
Neurobasal™-A Medium
Gibco™ DMEM/F-12, HEPES, no phenol red
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM/F-12, HEPES, No Phenol Red is used in human ex vivo tissue and cell culture processing applications.
Gibco™ IMDM, no phenol red
Suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
Gibco™ Recovery™ Cell Culture Freezing Medium
Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3
Gibco™ DMEM, powder, high glucose, pyruvate
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, Powder, High Glucose, Pyruvate, No Sodium Bicarbonate Buffer is used in cell culture processing applications.
Gibco™ CTS™ AIM-V™ Medium, without phenol red, without antibiotics
The first commercially available serum-free formulation for proliferation and/or manipulation of T cells, dendritic cells, and other primary or immortalized cells
Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES
Originally developed to culture human leukemic cells in suspension and as a monolayer
Cell culture media support the growth and maintenance of a variety of mammalian and other cell lines in vitro, as either anchorage-dependent or suspension cultures. Cell culture offers convenience, control, consistency and reproducibility of results, especially when using clonal cells.
Cell culture has multiple applications in cellular and molecular biology:
- Studying normal cell physiology and biochemistry
- Industrial bioprocess/bioreactor manufacturing of vaccines, therapeutic proteins and monoclonal antibodies using recombinant, high-yield protein expression systems
- Pharmaceutical screening and development, including drug metabolism and toxicology
- Other emerging applications in stem cell culture, immunotherapy and cell therapy
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
- Classical mammalian cell culture media (DMEM, MEM, RPMI 1640, McCoy’s, Ham’s, BME and EBSS) contain all the nutrients that cells need, such as amino acids, minerals, vitamins and glucose
- Individual cell culture reagents and supplements, including growth factors (e.g. pyruvate), antibiotics, hormones, cryopreservation media, cell dissociation reagents and animal-derived sera (e.g., FBS foetal bovine serum) which contain a ready-made natural mixture of nutrients
- Speciality media, including stem cell culture media, transfection media, and serum-free or animal-derived component-free (ACDF) additives
- Ancillary buffers and process liquids, including balanced salt solutions (PBS phosphate buffered saline, Hank’s HBSS, Earle’s EBBS), sodium bicarbonate and HEPES buffers, phenol red, cell culture grade water, and adherent cell microcarriers
Cell culture media are typically supplied as presterilised ready-to-use liquids, concentrates, powders or other solids in bottles, or else as single-use sealed bags for integration into compatible cell culture harvesting systems.
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories: