Crosslinking, marcatura e modificazione proteica

Thermo Scientific™ Soluzione di guanidina·HCl Pierce™ 8M

Thermo Scientific™ Soluzione di guanidina·HCl Pierce™ 8M

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Thermo Scientific™ DTT (Ditiotreitolo) Pierce™

Thermo Scientific™ DTT (Ditiotreitolo) Pierce™

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-biotina e kit di marcatura

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-biotina e kit di marcatura

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Thermo Scientific™ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

Thermo Scientific™ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))

Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.

Thermo Scientific™ MS(PEG)12 Methyl-PEG-NHS-Ester Reagent

Thermo Scientific™ MS(PEG)12 Methyl-PEG-NHS-Ester Reagent

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).

Thermo Scientific™ Reagenti Pierce™ metil-PEG-NHS-estere

Thermo Scientific™ Reagenti Pierce™ metil-PEG-NHS-estere

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).

Thermo Scientific™ DTNB Pierce™ (reagente di Ellman)

Thermo Scientific™ DTNB Pierce™ (reagente di Ellman)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Thermo Scientific™ Reagenti Pierce™ metil-PEG-NHS-estere

Thermo Scientific™ Reagenti Pierce™ metil-PEG-NHS-estere

Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).

Thermo Scientific™ EZ-Link™ NHS-LC-biotina

Thermo Scientific™ EZ-Link™ NHS-LC-biotina

Biotinylate protein amines (e.g., lysines) with this long-chain (22.4A), NHS-ester biotinylation reagent containing a membrane permeable spacer arm for intracellular labeling.

Thermo Scientific™ Gel a TCEP immobilizzato riducente il disolfuro Pierce™

Thermo Scientific™ Gel a TCEP immobilizzato riducente il disolfuro Pierce™

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Molecular Probes™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)

Molecular Probes™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)

Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)

Thermo Scientific™ N-etilmaleimide (NEM) Pierce™

Thermo Scientific™ N-etilmaleimide (NEM) Pierce™

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

Thermo Scientific™ Crosslinker Pierce™ BS(PEG)n

Thermo Scientific™ Crosslinker Pierce™ BS(PEG)n

Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.

Thermo Scientific™ Soluzione TCEP a pH neutro Bond-Breaker™

Thermo Scientific™ Soluzione TCEP a pH neutro Bond-Breaker™

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Thermo Scientific™ Pierce™ Alkylating Reagents

Thermo Scientific™ Pierce™ Alkylating Reagents

Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.

Cytiva Cy™3B Mono-reactive NHS Ester Fluorescent Dyes

Cytiva Cy™3B Mono-reactive NHS Ester Fluorescent Dyes

Combines exceptional photostability with bright and intense signals. Cytiva Cy™3B NHS Ester Mono-Reactive CyDye™ provides targeted and specific labeling of amine residues on antibodies, peptides, or oligonucleotides in fluorescence polarization applications.

Thermo Scientific™ MT(PEG)4 Methyl-PEG-Thiol Compound

Thermo Scientific™ MT(PEG)4 Methyl-PEG-Thiol Compound

MT(PEG)n: methyl and sulfhydryl compound with a 4-unit polyethylene glycol (PEG) spacer; methylate and pegylate quantum dots, gold surfaces, silver.

Thermo Scientific™ CA(PEG)4 Carboxy-PEG-Amine Compound

Thermo Scientific™ CA(PEG)4 Carboxy-PEG-Amine Compound

CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.

Thermo Scientific™ Tryptic Digestion Kits

Thermo Scientific™ Tryptic Digestion Kits

Improve peptide digest quality and sensitivity of MS detection using thesw Thermo Scientific Digestion Kits for efficient and reproducible preparation of peptide digests.

Molecular Probes™ Qdot™ 800 ITK™ Organic Quantum Dots

Molecular Probes™ Qdot™ 800 ITK™ Organic Quantum Dots

Qdot™ 800 ITK™ Organic Quantum Dots

Molecular Probes™ Biotin-4-Fluorescein

Molecular Probes™ Biotin-4-Fluorescein

Biotin-4-fluorescein can be used to quantitate available biotin-binding sites or the concentration of avidin or streptavidin through the strong quenching associated when this molecule binds to avidin.

Thermo Scientific™ GlycoLink™ Coupling Catalyst

Thermo Scientific™ GlycoLink™ Coupling Catalyst

Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.

Thermo Scientific™ EZ-Link™ Iodoacetyl-LC-Biotin

Thermo Scientific™ EZ-Link™ Iodoacetyl-LC-Biotin

Biotinylate reduced cysteines (-SH groups) at high pH via irreversible thioether bonds with this haloacetyl-activated, sulfhydryl-reactive biotinylation reagent.

Molecular Probes™ Alexa Fluor™ 488 Oligonucleotide Amine Labeling Kit

Molecular Probes™ Alexa Fluor™ 488 Oligonucleotide Amine Labeling Kit

Alexa Fluor™ 488 Oligonucleotide Amine Labeling Kit

Thermo Scientific™ EZ-Link™ Iodoacetyl-PEG2-Biotin

Thermo Scientific™ EZ-Link™ Iodoacetyl-PEG2-Biotin

Biotinylate reduced cysteines (-SH groups) at high pH with this haloacetyl-activated, sulfhydryl-reactive biotinylation reagent containing polyethylene glycol for solubility.

Thermo Scientific™ NeuCode™ Lysine-080

Thermo Scientific™ NeuCode™ Lysine-080

Increase the level of multiplexing achievable with NeuCode™ amino acids for the metabolic labeling of proteins for mass spectrometry analysis.

Thermo Scientific™ Pierce™ Iodination Tubes

Thermo Scientific™ Pierce™ Iodination Tubes

Reliably label (iodinate) proteins and peptides with iodine-125 using glass test tubes that are pre-coated (activated) with iodination reagent.

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