Crosslinking, marcatura e modificazione proteica
Thermo Scientific™ Soluzione di guanidina·HCl Pierce™ 8M
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Thermo Scientific™ DTT (Ditiotreitolo) Pierce™
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-biotina e kit di marcatura
Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.
Thermo Scientific™ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.
Thermo Scientific™ MS(PEG)12 Methyl-PEG-NHS-Ester Reagent
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ Reagenti Pierce™ metil-PEG-NHS-estere
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ DTNB Pierce™ (reagente di Ellman)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Thermo Scientific™ Reagenti Pierce™ metil-PEG-NHS-estere
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Thermo Scientific™ EZ-Link™ NHS-LC-biotina
Biotinylate protein amines (e.g., lysines) with this long-chain (22.4A), NHS-ester biotinylation reagent containing a membrane permeable spacer arm for intracellular labeling.
Thermo Scientific™ Gel a TCEP immobilizzato riducente il disolfuro Pierce™
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Molecular Probes™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Thermo Scientific™ N-etilmaleimide (NEM) Pierce™
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Thermo Scientific™ Crosslinker Pierce™ BS(PEG)n
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientific™ Soluzione TCEP a pH neutro Bond-Breaker™
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ Pierce™ Alkylating Reagents
Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.
Cytiva Cy™3B Mono-reactive NHS Ester Fluorescent Dyes
Combines exceptional photostability with bright and intense signals. Cytiva Cy™3B NHS Ester Mono-Reactive CyDye™ provides targeted and specific labeling of amine residues on antibodies, peptides, or oligonucleotides in fluorescence polarization applications.
Thermo Scientific™ MT(PEG)4 Methyl-PEG-Thiol Compound
MT(PEG)n: methyl and sulfhydryl compound with a 4-unit polyethylene glycol (PEG) spacer; methylate and pegylate quantum dots, gold surfaces, silver.
Molecular Probes™ Fluorescein-5-Thiosemicarbazide
fluorescein-5-thiosemicarbazide
Molecular Probes™ Alexa Fluor™ 488 TFP ester
Alexa Fluor™ 488 TFP ester
Thermo Scientific™ CA(PEG)4 Carboxy-PEG-Amine Compound
CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.
Thermo Scientific™ Tryptic Digestion Kits
Improve peptide digest quality and sensitivity of MS detection using thesw Thermo Scientific Digestion Kits for efficient and reproducible preparation of peptide digests.
Molecular Probes™ Qdot™ 800 ITK™ Organic Quantum Dots
Qdot™ 800 ITK™ Organic Quantum Dots
Molecular Probes™ Biotin-4-Fluorescein
Biotin-4-fluorescein can be used to quantitate available biotin-binding sites or the concentration of avidin or streptavidin through the strong quenching associated when this molecule binds to avidin.
Thermo Scientific™ GlycoLink™ Coupling Catalyst
Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.
Thermo Scientific™ EZ-Link™ Iodoacetyl-LC-Biotin
Biotinylate reduced cysteines (-SH groups) at high pH via irreversible thioether bonds with this haloacetyl-activated, sulfhydryl-reactive biotinylation reagent.
Molecular Probes™ Alexa Fluor™ 488 Oligonucleotide Amine Labeling Kit
Alexa Fluor™ 488 Oligonucleotide Amine Labeling Kit
Thermo Scientific™ EZ-Link™ Iodoacetyl-PEG2-Biotin
Biotinylate reduced cysteines (-SH groups) at high pH with this haloacetyl-activated, sulfhydryl-reactive biotinylation reagent containing polyethylene glycol for solubility.
Thermo Scientific™ NeuCode™ Lysine-080
Increase the level of multiplexing achievable with NeuCode™ amino acids for the metabolic labeling of proteins for mass spectrometry analysis.
Thermo Scientific™ Pierce™ Iodination Tubes
Reliably label (iodinate) proteins and peptides with iodine-125 using glass test tubes that are pre-coated (activated) with iodination reagent.