Protein Extraction and Purification

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophilic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.

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SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

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Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

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Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

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Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagents

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

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GE Healthcare Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000.  GE Healthcare Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

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Invitrogen™ Molecular Probes™ EZQ™ Protein Quantitation Kit

Fluorescence-based protein assay facilitates fast quantitation of protein samples prepared for gel electrophoresis

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Corning™ Spin-X™ UF Concentrators

Disposable, single use only ultrafiltration devices with polyethersulfone membranes (PES) for the centrifugal concentration and/or purification of biological fluids and aqueous solutions

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Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.

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Thermo Scientific™ anti-HA Epitope Tag Clone: 2-2.2.14

HA Epitope Tag Monoclonal antibody 26181 from Thermo Scientific™ specifically detects HA Epitope Tag in Tag samples, and it is validated for Immunoprecipitation

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Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

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GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.

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Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

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GE Healthcare Superdex™ 75 Prep Grade

Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins

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Thermo Scientific™ Pierce™ Centrifuge Columns

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

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Invitrogen™ DYNAL™ DynaMag™ 50 Magnet

Optimized for efficient cell isolation using Dynabeads™

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Thermo Scientific™ Pierce™ IP Lysis Buffer

Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent.

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Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

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Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads

Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups.

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Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

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Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

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Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

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Thermo Scientific™ NP-40 Detergent Surfact-Ams™ Solution

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

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Thermo Scientific™ Pierce™ Detergent Removal Spin Columns and Plates

Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing.

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GE Healthcare Streptavidin HP MultiTrap™ Plates

Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates

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GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

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GE Healthcare Thrombin Protease

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

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