Enzymes and Inhibitors
Thermo Scientific™ BclI
Cut at T^GATCA sites with BclI restriction enzyme, which performs best at 55°C in G buffer (Isoschizomers: FbaI, Ksp22I). BCLI 10U/UL 3000U
Thermo Scientific™ PstI
The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). PSTI 10U/UL 10000U
Thermo Scientific™ Eco47I (AvaII)
The Eco47I (AvaII) restriction enzyme recognizes G^GWCC sites and cuts best at 37°C in R buffer (Isoschizomers: AvaII, Bme18I, SinI, VpaK11BI). ECO47I (AVAII) 10U/UL 800U
OPTIZYME™ SmaI, Fisher BioReagents™
Optizyme Sma I restriction enzyme
Thermo Scientific™ Hin1II (NlaIII)
Cut at CATG^ sites with Hin1II (NlaIII) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: CviAII, FaeI, FatI, Hsp92II, NlaIII). 300 UNIT RESTRICTION ENZYME, CONVENTIONAL, HIN1II(NlaIII),5u/ul, cuts CATG¬ at 37°C (supplied with
Thermo Scientific™ Hin1I (BsaHI)
Cut at GR^CGYC sites with Hin1I (BsaHI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: AcyI, BsaHI, BssNI, BstACI, Hsp92I). RESTRICTION ENZYME, CONVENTIONAL, HIN1I (BSAHI),10u/uL, cuts GR¬CGYC at 37°C (supplied with 1mL
Thermo Scientific™ FastDigest HinfI
Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 400 UL RESTRICTION ENZYME, FASTDIGEST, HINFI,cuts G¬ANTC, 1µL cleaves 1µg DNA in5 minutes at
Thermo Scientific™ Kpn2I (BspEI)
The Kpn2I (BspEI) restriction enzyme recognizes T^CCGGA sites and cuts best at 55°C in Tango buffer (Isoschizomers: AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI). KPN2I (BSPEI) 10U/UL 500U
Thermo Scientific™ EcoRI
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer. ECORI 50U/UL 25000U
Thermo Scientific™ BoxI (PshAI)
Cut at GACNN^NNGTC sites with BoxI (PshAI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BstPAI, PshAI). RESTRICTION ENZYME, CONVENTIONAL, BOXI (PSHAI),10u/ul, cuts GACNN¬NNGTC at 37°C (supplied with
Thermo Scientific™ FastDigest PmlI (Eco72I)
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. Restriction enzyme, FastDigest Eco72I, 200µL
Thermo Scientific™ Alw26I (BsmAI)
Cut at GTCTC(1/5)^ sites with Alw26I (BsmAI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsmAI, BstMAI). RESTRICTION ENZYME, CONVENTIONAL, ALW26I, 10U/µLcuts GTCTC(1/5)¬ at 37°C (supplied with 1mL of
OPTIZYME™ SphI (PaeI), Fisher BioReagents™
Optizyme Sph I restriction enzyme
Thermo Scientific™ AdeI (DraIII)
Cut at CACNNN^GTG sites with AdeI (DraIII) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: DraIII). RESTRICTION ENZYME, CONVENTIONAL, ADEI (DRAIII),10u/ul, cuts CACNNN¬GTG at 37°C (supplied with
Thermo Scientific™ Bsh1236I (BstUI)
Cut at CG^CG sites with Bsh1236I (BstUI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI). BSH1236I (BSTUI) 10U/UL 500U
Thermo Scientific™ Buffer G
Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer G, premixed with BSA for enhanced stability. X10 BUFFER G 5X1ML
OPTIZYME™ AarI, Fisher BioReagents™
Optizyme Aar I restriction enzyme
Thermo Scientific™ Buffer EcoRI
Optimize digestions with EcoRI restriction enzyme using this buffer, premixed with BSA for enhanced stability. X10 BUFF ECORI 5X1ML
Thermo Scientific™ DraI
Cut at TTT^AAA sites with DraI restriction enzyme, which performs best at 37°C in Tango buffer. 1500 UNIT RESTRICTION ENZYME, CONVENTIONAL, DRAI,10u/uL, cuts TTT¬AAA at 37°C (suppliedwith 1mL of
Thermo Scientific™ FastDigest BstXI
Cut at CCANNNNN^NTGG sites with Thermo Scientific™ FastDigest™ BstXI, which performs best at 55°C in 5-15 minutes using universal FastDigest Buffer. 100 UL RESTRICTION ENZYME, FASTDIGEST, BSTXI,cuts CCANNNNN¬NTGG, 1µL cleaves 1µgDNA in 5 minute
Thermo Scientific™ FastDigest Tru1I
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 50 UL RESTRICTION ENZYME, FASTDIGEST, TRU1I, CUTST¬TAA,1µL cleaves 1µg DNA in 5 minutes at 65°C
Thermo Scientific™ FastDigest Eco91I
Cut at G^GTNACC sites with Thermo Scientific™ FastDigest™ Eco91I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. FASTDIGEST ECO91I 200UL
Thermo Scientific™ FastDigest Bpu10I
Cut at CCTNAGC(-5/-2)^ sites with Thermo Scientific™ FastDigest™ Bpu10l, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. 20 UL RESTRICTION ENZYME, FASTDIGEST, BPU10I,cuts CCTNAGC(-5/-2)¬, 1µL cleaves 1µg DNA in 15
Thermo Scientific™ FastDigest™ NdeI
The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI). 300 UL RESTRICTION ENZYME, FASTDIGEST, NDEI, CUTSCA¬TATG, 1µL cleaves 1µg DNA in 5 minutes at 37°C
Thermo Scientific™ MspI (HpaII)
The MspI (HpaII) restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer. MSPI (HPAII) 10U/UL 5X3000U
Thermo Scientific™ TaaI (HpyCH4III)
The TaaI (HpyCH4III) restriction enzyme recognizes ACN^GT sites and cuts best at 65°C in Tango buffer (Isoschizomers: Bst4CI, HpyCH4III). 200 UNIT RESTRICTION ENZYME, CONVENTIONAL, TAAI(HpyCH4III), 10u/ul, cuts ACN¬GT at 65°C (supplied
Thermo Scientific™ BplI
Cut at ^(8/13)GAG(N)5CTC(13/8)^ sites with BplI restriction enzyme, which performs best at 37°C in Tango(+SAM) buffer. BPLI 5U/UL 500U
Thermo Scientific™ FastDigest AseI (VspI)
Cut at AT^TAAT sites with Thermo Scientific™ FastDigest™ AseI (VspI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PshBI). Restriction enzyme, FastDigest VspI, 200µL
Thermo Scientific™ FastDigest MspI
Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 400 UL RESTRICTION ENZYME, FASTDIGEST, MSPI, CUTSC¬CGG, 1µL cleaves 1µg DNA in 5 minutes at 37°C
Thermo Scientific™ HpaII
The HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer. HPAII 10U/UL 1000U