Dissociation Reagents

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression. 5ML Matrigel Basement Membrane Matrix, LDEV-Free

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells INSULIN-TRANS-SEL-G, 100X

Gibco™ ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow ACK LYSING BUFFERACK Lysing Buffer is suitable for use in the

Lonza™ BioWhittaker™ L-Glutamine (200mM)

Manufactured in accordance with cGMP regulations L-Glutamine 200mM 50mL

Gibco™ Pluronic F-68 Non-ionic Surfactant (100X)

A non-ionic surfactant used to control shear forces in suspension cultures PLURONIC

Gibco™ GlutaMAX™ Supplement

An alternative to L-glutamine, with increased stability that improves cell health 100ML GlutaMAX Supplement

Corning™ Fibronectin, Human

Promotes cellular attachment, spreading, and proliferation on tissue-culture surfaces. Corning™ Fibronectin, Human can be used as an additive to serum-free culture media. 1MG Fibronectin, Human

Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis. 10MG Cell-Tak Cell and Tissue Adhesive

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression. 5ML Matrigel Growth Factor Reduced (GFR) Basement

Invitrogen™ Molecular Probes™ Low Density Lipoprotein from Human Plasma, DiI complex (DiI LDL)

Nonacetylated conjugate LOW-DENSITY LIPOPROTEIN Fcomplex (DiI LDL) 1 mg/ml, 200 µl

Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media HEPES (1M) 100ML

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses 100ML Cell Recovery Solution

Gibco™ L-Glutamine (200mM)

Ready-to-use stock solution that acts as a cell culture supplement 20ML L GLUTAMINE, 100X

Gibco™ Sodium Pyruvate (100mM)

Growth supplement 100ML Sodium Pyruvate

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test TRYPAN BLUE STAIN

Invitrogen™ Molecular Probes™ Zymosan A S. cerevisiae BioParticles™ , unlabeled

Nonfluorescent products are useful in phagocytosis assays, both as controls and for custom labeling with reactive dyes ZYMOSAN A (S. CEREVISIAE)The unlabeled zymosan A BioParticles® product is

Lonza™ BioWhittaker™ Trypsin Reagent

Used to remove cells from the culture vessel surface into suspension.  BioWhittaker™ Trypsin Reagentis porcine parvovirus tested and mycoplasma screened Trypsin 1:250(10X)2.5% in Hanks'BSS w/o Ca++ 100ml

Lonza™ BioWhittaker™ Dulbeccos Phosphate Buffered Saline (10X)

Used as a buffer system for cell culture media.  BioWhittaker™ Dulbeccos Phosphate Buffered Saline (10X) is manufactured in accordance with cGMP regulations and cell culture-tested to assure compatibility. DPBS (10X) w/o Ca++ or Mg++, 500 ml

Gibco™ Sterile Distilled Water

6L DISTILLED WATER (6x1000ml)

Invitrogen™ Molecular Probes™ Zymosan A (S. cerevisiae) BioParticles™ , Alexa Fluor™ 488 conjugate

Study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry ZYMOSAN A (S. CEREVISIAE)The Alexa Fluor® 488 zymosan A BioParticles®

Gibco™ 2-Mercaptoethanol (55mM)

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals 2 MERCAPTOETHANOL 1000X

Corning™ BioCoat™ Poly-D-Lysine

Recommended for the culture of neurons, glial cells, and transfected cells 20MG BioCoat Poly-D-Lysine

Lonza™ BioWhittaker™ MEM Eagle Nonessential Amino Acid Solution (100X)

Used to enhance cell growth. BioWhittaker™ MEM Eagle Nonessential Amino Acid Solution (100X) is manufactured in accordance with cGMP regulations. MEM NEAA (10 mM, 100X 100 ml

Lonza™ BioWhittaker™ Hank's Balanced Salt Solutions (1X)

Used in wide variety of tissue culture applications. BioWhittaker™ Hank's Balanced Salt Solutions (1X) is used to maintain a physiological pH for cells maintained in non-CO2 conditions, diluting cells before counting, washing cells before dissociating and transporting cells or tissues. Hanks' BSS (1X) w/o Ca++,Mg++, phenol red, 500ml

Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability 100 ML MEM NON-ESSENTIAL AMINO ACIDS SOLUTION10mM (100X), liquid 100mL RRR - pack at ambient

Corning™ Nu-Serum™ Growth Medium Supplement

Provides a low-serum alternative to newborn calf, fetal bovine, and other sera used for cell culture 100ML Nu-Serum Growth Medium Supplement

Invitrogen™ Molecular Probes™ Zymosan A S. cerevisiae BioParticles™ , Fluorescein conjugate

Study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry ZYMOSAN A (S. CEREVISIAE)The fluorescein zymosan A BioParticles®

Invitrogen™ Molecular Probes™ Transferrin From Human Serum, Alexa Fluor™ 568 Conjugate

Labeled LDL complexes useful in studying the delivery of up to two Fe3+ atoms via receptor-mediated endocytosis TRANSFERRIN FROM HUMAN SEThe red-fluorescent Alexa Fluor 568 conjugate of

MP Biomedicals™ Accutase

ACCUTASE CELL DETACHMENT SOLUTION 100ML

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