Biological Buffers
Invitrogen™ Nuclease-Free Water (not DEPC-Treated)
1 LT NUCLEASE-FREE WATER (NOT DEPC-TREATED) 1000MLStore at room temperature
Thermo Scientific™ 6X DNA Loading Dye
Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye. X5 6X DNA LOADING DYE, COMPOSITION: 10MM TRIS-HCL(pH7.6), 0.03% bromophenol blue, 0.03% xylene
Invitrogen™ Potassium Acetate (3M), pH 5.5
Ready-to-use, certified RNase-free 100 ML 3 M POTASSIUM ACETATE, PH 5.5 100ML STOREat room temperature
GE Healthcare Pharmalyte™ Carrier Ampholytes
Use in Isoelectric focusing. GE Healthcare Pharmalyte™ Carrier Ampholytes prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in five broad-range and four narrow-range pH intervals. 25ML Pharmalyte, broad range pH 2.5 - 5Store in Fridge at 4C
Invitrogen™ EDTA (0.5M), pH 8.0
Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature
Invitrogen™ Tris (1M), pH 8.0
Ready-to-use, certified RNase-free 500 ML 1 M TRIS, PH 8.0 500ML STORE AT ROOM TEMPERature
Invitrogen™ Novex™ Tris-Glycine SDS Running Buffer (10X)
Formulated for separation of proteins, in their denatured state, on Novex Tris-Glycine gels TS SDS 10X RUN BUF, 5L
Laemmli SDS Sample Buffer, reducing, 6X
For protein sample preparation to be used in the Laemmli SDS-PAGE system 50ML Laemmli SDS sample buffer, reducing (6X) 50ml
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 100GR HEPES (Fine White Crystals) for Molecular Biology,
Invitrogen™ Novex™ 4X Bolt™ LDS Sample Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats BOLT 4X LDS SAMPLE BUFFER
Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels)
Provide separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer TRIS-ACETATE BUFFER KIT, NUPAGE(R) 00
Invitrogen™ Novex™ 20X Bolt™ MES SDS Running Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 500 ML BOLT MES SDS RUNNING BUFFR 20X
Thermo Scientific™ Blocker™ BSA Blocking Buffers
Block membranes, plates and other samples with these ready-to-use 10X PBS or TBS solutions of bovine serum albumin for Western blot, ELISA and other detection methods. BLOCKER BSA IN TBS(10X)125ML
Invitrogen™ BSA, acetylated (20 mg/mL)
Stabilizes enzymatic reactions to ensure the absence of contaminutesants ACETYLATED BSA (20MG/ML)6X25MGAmbion® Acetylated BSA is used as a carrier
Thermo Scientific™ Restore™ Plus Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates. X2 RESTORE(TM) PLUS WESTERN BLOT STRIPPING BUFFER30mL
Invitrogen™ Novex™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis 250 UL SAMPLE REDUCING AGENT (10X), LIQUID NUPAGE(r) 250µL store at 2° to 8°C
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents
Commonly used buffering agent 1LT HEPES Buffer, 1M Solution, pH 7.3, for Molecular Biology
Invitrogen™ Novex™ Tris-Glycine Transfer Buffer (25X)
Made with high-purity reagents and are strictly quality controlled TRISGLY TRANS BUF (25X)500MLNovex® Tris-Glycine Transfer Buffer (25X) is
Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X)
LDS SAMPLE BUFFER (4X), NUPAGE(R) 250ML STORE AT2-8C
Invitrogen™ Sodium Acetate (3M), pH 5.5
Ready-to-use, certified RNase-free 100 ML 3 M SODIUM ACETATE, PH 5.5 100ML STORE ATroom temperature
Invitrogen™ Novex™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats 10 ML BOLT SAMPLE REDUCING AGENT
Thermo Scientific™ MgCl2 (Magnesium Chloride)
Optimize your PCR reaction conditions with this Magnesium Chloride (MgCl2) solution. 5ML 25MM MGCL2
Invitrogen™ Proteinase K
Remains active under various conditions PROTEINASE K 100MG
Invitrogen™ AccuPrime™ Pfx DNA Polymerase
Provides higher fidelity than Pfu DNA polymerase 1000 RXN PFX DNA POLYMERASE ACCUPRIME(TM) 1000reactions Store at -20C
Alfa Aesar™ HEPES hemisodium salt
CAS: 103404-87-1 Molecular Formula: C8H17N2NaO4S Molecular Weight (g/mol): 260.284 MDL Number: MFCD00036463 InChI Key: RDZTWEVXRGYCFV-UHFFFAOYSA-M Synonym: 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium salt PubChem CID: 2724248 ChEBI: CHEBI:46758 IUPAC Name: sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate SMILES: C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+] 500GR HEPES hemisodium salt
Lonza™ Ficoll™ DNA Loading Buffer, 6X
Ideal for improving resolution and separation of DNA fragments DNA Loading Buffer 6X FP=5 x 1 ml
Invitrogen™ Novex™ NuPAGE™ Antioxidant
Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis 15ML NUPAGE ANTIOXIDANT
Invitrogen™ Novex™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels NUPAGE TA SDS RNBUF (20X)
Invitrogen™ Proteinase K Solution (20mg/mL)
General digestion of proteins PROTEINASE K (20MG/ML)5X1.25ML5 tubes