Antibotics, Buffers and Supplements

Gibco™ Trypsin-EDTA (0.25%), phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Gibco™ Trypsin (2.5%), no phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ Trypsin-EDTA (0.05%), phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ 2-Mercaptoethanol

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Gibco™ Phytohemagglutinin, M form (PHA-M)

This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1 to 2% v/v

Gibco™ Pluronic™ F-68 Non-ionic Surfactant (100X)

A non-ionic surfactant used to control shear forces in suspension cultures

Gibco™ KaryoMAX™ Giemsa Stain Solution

For G-banding of chromosomes for cytogenetic analysis

Corning™ DMSO (Dimethyl Sulfoxide)

For cryopreservation of cells along with glycerol, to prevent damage to cell membrane during freezing

Gibco™ Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix

Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix

Gibco™ GlutaMAX™ Supplement

An alternative to L-glutamine, with increased stability that improves cell health

Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ L-Glutamine (200 mM)

Ready-to-use stock solution that acts as a cell culture supplement

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix

Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix

Corning™ Trypan Blue

Can be used for variety cell counting methods requiring information on cell count and viability.

Gibco™ Bacto™ Tryptone

For use with dehydrated media culture

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red

Unlike porcine trypsin, free of animal-derived components

Gibco™ HAT Supplement (50X)

HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells

Corning™ Matrigel™ Membrane Matrix HC

High concentration (HC) formulation is ideal for applications in which a high protein concentration augments the growth of tumors; allows a Matrigel basement membrane matrix plug to maintain its integrity after subcutaneous injection into mice

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O

Invitrogen™ Tryptose Phosphate Broth

Cell culture supplement for BHK-21 cells used for vaccine production

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Gibco™ Bacto™ Peptone

Enzymatic digest of protein

Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Gibco™ TrypLE™ Express Enzyme (1X), phenol red

Animal origin-free, recombinant enzyme

Gibco™ Trypsin Neutralizer Solution

Sterile, phosphate-buffered saline solution

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

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