Enzymes and Inhibitors

Thermo Scientific™ Bovine Serum Albumin (BSA)

Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin. 250 UL BOVINE SERUM ALBUMIN (BSA), 20MG/ML, 5MG(0.25mL), supplied in 10 mM Tris-HCl (pH7.4 at

Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA)

Perform electrophoresis of nucleic acids in agarose and polyacrylamide gels using Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA). TBE BUFFER 1L 10X

Thermo Scientific™ MvaI (BstNI)

Cut at CC^WGG sites with MvaI (BstNI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI). 2000 UNIT RESTRICTION ENZYME, CONVENTIONAL, MVAI(BstNI), 10u/uL, cuts CC¬WGG at 37°C (supplied

Thermo Scientific™ FastDigest TaqI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer. 400 UL RESTRICTION ENZYME, FASTDIGEST, TAQI, CUTST¬CGA, 1µL cleaves 1µg DNA in 5 minutes at 65°C

Thermo Scientific™ AarI

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer. 25 UNIT RESTRICTION ENZYME, CONVENTIONAL, AARI,2u/ul, 25 u, cuts CACCTGC(4/8)¬ at 37°C (supplied

Thermo Scientific™ BsuRI (HaeIII)

Cut at GG^CC sites with BsuRI (HaeIII) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI). RESTRICTION ENZYME, CONVENTIONAL, BSURI (HAEIII),10u/µL cuts GG¬CC at 37°C (supplied with 1mL of

Thermo Scientific™ BshTI (AgeI)

Cut at A^CCGGT sites with BshTI (AgeI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AgeI, AsiGI, CspAI, PinAI). BSHTI (AGEI) 10U/UL 1000U

Thermo Scientific™ XagI (EcoNI)

Cut at CCTNN^NNNAGG sites with XagI (EcoNI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BstENI, EcoNI). XAGI (ECONI) 10U/UL 1000U

Thermo Scientific™ FastDigest Bsp1286I (SduI)

Cut at GDGCH^C sites with Thermo Scientific™ FastDigest™ Bsp1286I (SduI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MhlI). Restriction enzyme, FastDigest SduI, 50µL

Thermo Scientific™ FastDigest EcoNI (XagI)

Cut at CCTNN^NNNAGG sites with Thermo Scientific™ FastDigest™ EcoNI (XagI), which performs best using universal FastDigest Buffer (Isoschizomers: BstENI). Restriction enzyme, FastDigest XagI, 100µL

Thermo Scientific™ FastDigest™ SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI) 200 UL RESTRICTION ENZYME, FASTDIGEST, SACI, CUTSGAGCT¬C, 1µL cleaves 1µg DNA in 5 minutes at 37°C

Thermo Scientific™ BamHI

The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer. X5 BAMHI 10U/UL 4000U

Thermo Scientific™ FastDigest AluI

Cut at AG^CT sites with Thermo Scientific™ FastDigest™ AluI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AluBI). 100 UL RESTRICTION ENZYME, FASTDIGEST, ALUI, CUTSAG¬CT, 1µL cleaves 1µg DNA in 15 minutes at 37°C

Thermo Scientific™ Buffer Cfr9I

Optimize digestions with Cfr9I restriction enzyme using this buffer, premixed with BSA for enhanced stability. 10X BUFF CFR9I 1ML

Thermo Scientific™ PaeI (SphI)

The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI). PAEI (SPHI) 10U/UL 2500U

Thermo Scientific™ FastDigest HpaII

Perform single-, double- or multiple DNA digestion within 5-15 minutes withthis advanced line of restriction enzymes, 100% active in one universal buffer. 200 UL RESTRICTION ENZYME, FASTDIGEST, HPAII,cuts C¬CGG,1µL cleaves 1µg DNA in 5 minutes at

Thermo Scientific™ Buffer BfuI

Optimize digestions with BfuI restriction enzyme using this buffer, premixed with BSA for enhanced stability. 10X BUFFER BFUI 1ML

Thermo Scientific™ Alw44I

Cut at G^TGCAC sites with Alw44I (ApaLI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: ApaLI, VneI). ALW44I 10U/UL 1000U

OPTIZYME™ XhoI, Fisher BioReagents™

Optizyme Xho I restriction enzyme

Thermo Scientific™ BclI

Cut at T^GATCA sites with BclI restriction enzyme, which performs best at 55°C in G buffer (Isoschizomers: FbaI, Ksp22I). BCLI 10U/UL 5000U

Thermo Scientific™ FastDigest BstXI

Cut at CCANNNNN^NTGG sites with Thermo Scientific™ FastDigest™ BstXI, which performs best at 55°C in 5-15 minutes using universal FastDigest Buffer. 100 UL RESTRICTION ENZYME, FASTDIGEST, BSTXI,cuts CCANNNNN¬NTGG, 1µL cleaves 1µgDNA in 5 minute

Thermo Scientific™ SatI (Fnu4HI)

The SatI (Fnu4HI) restriction enzyme recognizes GC^NGC sites and cuts best at 37°C in G buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI). SATI (FNU4HI) 10U/UL 200U

Thermo Scientific™ FastDigest BspMI (BveI)

Cut at ACCTGC(4/8)^ sites with Thermo Scientific™ FastDigest™ BspMI (BveI), which performs best using universal FastDigest Buffer (Isoschizomers: Acc36I, BfuAI). Restriction enzyme, FastDigest BveI, 50µL

Thermo Scientific™ Hin1I (BsaHI)

Cut at GR^CGYC sites with Hin1I (BsaHI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: AcyI, BsaHI, BssNI, BstACI, Hsp92I). RESTRICTION ENZYME, CONVENTIONAL, HIN1I (BSAHI),10u/uL, cuts GR¬CGYC at 37°C (supplied with 1mL

Thermo Scientific™ BveI (BspMI)

Cut at ACCTGC(4/8)^ sites with BveI (BspMI) restriction enzyme, which performs best at 37°C in O (+oligo) buffer (Isoschizomers: Acc36I, BfuAI, BspMI). 250 UNIT RESTRICTION ENZYME, CONVENTIONAL, BVEI(BspMI), 5u/ul, cuts ACCTGC(4/8)¬ at 37°C (supplie

Thermo Scientific™ MluI

The MluI restriction enzyme recognizes A^CGCGT sites and cuts best at 37°C in R buffer. MLUI 10U/UL 1000U

Thermo Scientific™ Csp6I (CviQI)

Cut at G^TAC sites with Csp6I (CviQI) restriction enzyme, which performs best at 37°C in B buffer (Isoschizomers: AfaI, CviQI, RsaNI). 1500 UNIT RESTRICTION ENZYME, CONVENTIONAL, CSP6I(CviQI), 10u/uL, cuts G¬TAC at 37°C (supplied

Thermo Scientific™ Psp1406I (AclI)

The Psp1406I (AclI) restriction enzyme recognizes AA^CGTT sites and cuts best at 37°C in Tango buffer (Isoschizomers: AclI). PSP1406I (ACLI) 10U/UL 300U

Thermo Scientific™ Nb.Mva1269I

Nick at GAATGC sites with Nb.Mva1269I nicking enzyme, which performs best at 37°C in O buffer. RESTRICTION ENZYME, NICKING, NB.MVA1269I, 10U/UL,cuts CCTAC¬G (lower strand) at 37°C(supplied with

Thermo Scientific™ FastDigest FspI (NsbI)

Cut at TGC^GCA sites with Thermo Scientific™ FastDigest™ FspI (NsbI), which performs best using universal FastDigest Buffer (Isoschizomers: Acc16I, AviII). Restriction enzyme, FastDigest NsbI, 50µL

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