Application Page

Ready-to-use Protocols for DNA and RNA Isolation From any Sample

MP Biomedicals supplies a complete range of organic, spin column, and magnetic bead based extraction kits for the isolation of high quality DNA and RNA. All kits included in the FastPrep product line contain optimized lysing matrix tubes for the sample homogenization step and reagents for further DNA, RNA or protein isolation. Yields and processing times are greatly enhanced through the use of FastPrep homogenizers. 

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Flexible Magnetic-Bead Based Kits Specially Developed for Automated DNA and RNA Purification

Magbeads FastDNA and FastRNA kits are using magnetic beads technology with high binding capacity and selectivity for DNA and RNA to isolate nucleic acids. They include Lysing Matrix tubes for a thorough and rapid grinding of the sample.

High-quality nucleic acids isolated with the MagBeads Kits are suitable for a broad range of applications including real-time PCR and next-generation sequencing

• High quality DNA and RNA: Intact, high yield, and free from inhibitors
• Flexible: Compatible with a wide range of automated nucleic acid purification platforms
• Versatile: Dedicated kits for any specific application

7X™ - Cleaning Solutions

Trusted by labs worldwide and available exclusively from MP Biomedicals, 7X™ solutions have been used for over 50 years to provide safe and effective cleaning of laboratory benches and apparatus whenever a high degree of cleanliness is required. They are of particular value in cell culture laboratories where they have a number of advantages:

7X™ cleaning solutions should be of particular value in cell culture laboratories where

• Suitable for washing away DNase/RNase, bacteria, radioactive contaminants and sticky biologicals
• Suitable for manual washing & ultrasonic cleaning
• Suitable for automatic washers/sprayers
• Supplied as a convenient ready-to-use 5% solution

Fully Chemically Defined FBS Replacements

Fetal bovine serum (FBS) is widely used as a serum-supplement for in vitro cell culture media. FBS promotes healthy cell growth by providing an undefined mixture of nutrients, such as proteins, attachment factors, growth factors, lipids and hormones. However, due to its undefined nature, FBS can lead to unexpected and undesired stimulation of cells. There is also biorisk from animal protein or pathogen contamination, including risk of bovine spongiform encephalopathy (BSE). To avoid these concerns, MP Biomedicals™ has launched FastGro™, a fully chemically defined FBS replacement for cell culture use. It allows culturing of a wide range of cells in vitro without the use of serum or any animal or human derived compounds.

• Chemically defined nature without lot-to-lot variations 
• No animal or human derived materials or compounds 
• No interference with hormones or growth factors 
• Elimination of the risk of contaminants - viruses, mycoplasma, prions, etc
• Wide range of cell culture practices 
• Storage in the refrigerator – no need for thawing before use 

Encapsulated Media

The ultimate in consistency & accuracy, MP Biomedicals is well known for providing high-quality growth media in a variety of formulations for every molecular biologist. We specialize in formulations for yeast and bacteria and offer more than a thousand recipes and variations. MP Biomedicals is the only source of encapsulated growth media. Our patented media capsules eliminate the waste, inaccuracies, and mess associated with weighing out bulk powder. Each recipe is subjected to extensive quantitative testing, and is Molecular Biology Certified™ through qualitative tests such as cell density and plasmid yield. Easy to use. Simply drop the capsule in water and autoclave. No Mess. No Cleanup.

Because of its high efficacy, MP Biomedicals™ Premium DSS (MW = 36,000–50,000) is one of the most widely used DSS products in the scientific literature.

MP Biomedicals™ Premium DSS technical advantages:

• Induces severe colitis (inflamatory bowel disease)
• Highest sulfur content – 19%
• Highest chirality - +104° of specific rotation 
• Lowest pH – 6.2 at 1% solution

Agaroses

Agarose powders for electrophoresis:

• Basic Agarose Premier: Your first price agarose for daily gels & standard analysis. This agarose has high gel strength even at low concentrations. Ideal for routine and rapid separation of DNA and RNA fragments
• Agarose Standard (low EEO): The optimal agarose for analytical and preparative purpose. Agarose Standard is a very low electroendosmosis agarose (EEO) which is advised for sharp resolution of nucleic acid fragments greater than 1000 bp
• Agarose High Resolution: The perfect agarose for small DNA fragments. Agarose High Resolution is an intermediate melting and gelling point agarose with twice the resolution capabilities of routine agarose and presents superior sieving characteristics. Ideal for DNA fragments under 1000 bp
• Agarose Multipurpose (or High Gel Strength Agarose): This is the highest gel strength available, especially designed for a wide range of molecular biology techniques
• Agarose Low Melting Point: This Agarose has finer sieving characteristics than standard agaroses. The low melting temperature enables nucleic acid recovery without denaturation or damage. The agarose will remain in a liquid state at 37°C allowing gel manipulations without prior DNA purification