Cytiva

Cytiva Cytiva

Cytiva: speed, efficiency, capacity

Cytiva is a global provider of technologies and services that help advance and accelerate the development and manufacture of therapeutics. With a heritage of innovation, we continue to move forward, building on the brands you know and have come to rely on for quality and reliability. Our diverse portfolio includes well-recognised brands such as ÄKTA™, Amersham™, HyClone™, MabSelect™ and Whatman™. Cytiva brings speed, efficiency and capacity to life sciences research and manufacturing, enabling the delivery of transformative medicines to patients.

Protein Research

Choose from Cytiva's portfolio of protein purification tools, from Sephadex™, the chromatography resin that revolutionised protein purification, to the newest magnetic bead-based technology. Cytiva's products support a broad range of chromatography resins and resin methods, including affinity purification, gel filtration, desalting, ion exchange and hydrophobic interaction, all in formats to meet your application requirements.

  • Loose resin up to 500 g
  • Chromatography columns such as HiTrap™, SpinTrap™, GraviTrap™ and Tricorn™ columns
  • Pre-packed columns for convenience and efficiency

Western Blotting

Amersham™ Western blotting products deliver reliable protein identification for you time after time: meaningful data, reproducible results.


Key Products

Cell Culture

HyClone™ serum, media buffers and process liquids have helped advance drug discovery and bioproduction processes since 1967, and continue to deliver quality and consistency.

HyClone™ Classical and Serum-Free Media »

Culture cells using our complete line of HyClone™ classical, specialty and transfection media products that support cell growth of CHO, SF9, HEK293, hybridoma, myeloma, insect, NS0 and viral vaccine cell lines.

HyClone™ Fetal Bovine Serum (FBS), Calf Serum, Fetal Bovine Serum Alternatives, and Supplements »

Enhance cell culture performance using a variety of HyClone™ supplements, including Fetal Bovine Serum (FBS), calf serum, engineered serum (FetalClone™ 1, 2, 3) and serum-free supplements (HyClone™ Cell Boost 1-6).

HyClone™ Antibiotics and Reagents »

Separate attachment-dependent cells from substrate or reduce the effect of bacteria, mould or yeast contaminants in your cell culture process.

HyClone™ Stem Cell Products »

Grow stem cells with ease using our specialty line of stem cell products tailored to optimize cell culture performance for a variety of stem cells, including induced pluripotent stem cells (iPSC), mesenchymal stem cells (mSC), human embryonic stem cells (hESC) and epithelial stem cells.

HyClone™ Buffers and Process Liquids »

Manage, control and regulate pH, temperature, osmotic pressure, carbon dioxide and oxygen for your specific cell culture.

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Key Products

DNA and RNA Research

When nucleic acid (DNA or RNA) sample is scarce, amplification can be used to expand the sample quantity for use in multiple experiments. Selecting the most suitable amplification technology will depend on sample type, the molecular biology application where it will be used, and the need to preserve sample for additional testing. Some common technologies for DNA amplification are Phi29 polymerase technology, polymerase chain reaction (PCR), rolling circle amplification (RCA), and isothermal multiple strand displacement amplification (MDA).

Phi29 polymerase technology

Phi29 DNA polymerase-based isothermal DNA amplification is a simple, reliable alternative to other DNA amplification procedures such as polymerase chain reaction (PCR). The highly processive Phi29 DNA polymerase elicits strong strand displacement enabling rapid DNA replication from multiple sites. 

Whole genome amplification

GenomiPhi™ products, powered by Phi29 polymerase technology, are available in kits that provide an easy-to-use method which delivers highly representative and reliable whole genome amplification. Each kit contains all the components necessary for whole-genome amplification by utilising isothermal multiple strand displacement amplification, with minimal hands-on time. Compared with conventional PCR amplification techniques, MDA generates larger sized products with a lower error frequency.

Rolling circle amplification

TempliPhi™, based on Phi29 DNA polymerase, is used to exponentially amplify single- or double-stranded circular DNA by rolling circle amplification (RCA). This isothermal amplification produces microgram quantities of DNA from picograms of starting material in 4-6 h.


Whatman™ Filtration

Filtration is an indispensable step in sample preparation and many analytical methods. Cytiva's Whatman™ products provide the quality, consistency, reliability and convenience to help your lab run smoothly. With a large variety of available sizes, types and specifications, you can be sure to find a filter that fits your application, equipment and workflow.

  • Cellulose, glass and quartz fibre filters
  • Membrane filters
  • Syringe and syringeless filtration devices
  • Capsule filters
  • Sterile and non-sterile options
  • Lab essentials

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Whatman Puradisc syringe filter

  • High performance, dependable single membrane layer filter
  • Available in 11 different membrane types and seven pore sizes to work for samples of many chemical compositions and volumes

Whatman GD/X™ syringe filter

  • Used for heavy, thick, or viscous hard-to-filter samples
  • Multi-gradient glass-fibre layer pre-filters large particles which increases flow rate and volume through the main membrane

Whatman GD/XP syringe filter

  • Used for heavy, thick, or viscous samples with low protein binding and low metals extractables
  • Polypropylene pre-filtration stack provides increased flow rate and volume flow through while eliminating protein binding and inorganic ion extractables that could come from glass fibre pre-filtration 

Whatman SPARTAN™ certified

  • Every batch is tested and certified for HPLC use to ensure batch-to-batch consistency and low levels of UV-absorbing substances
  • Regenerated cellulose membrane exhibits very low protein binding and low levels of extractables in water, methanol and acetonitrile

Whatman Anotop™

  • Recommended for use in ion chromatography 
  • Alumina-based Anopore membrane has very low ion extractables and highly uniform capillary pore structure

Whatman Mini-UniPrep™ (MUP)

  • Designed for HPLC and uHPLC autosamplers to support high throughput testing labs
  • Combines syringe, syringe filter, autosampler vial, cap and septum in a single device for increased efficiency in consumable management

Whatman Mini-UniPrep™ (MUP) G2

  • Designed for HPLC and UHPLC autosamplers to support high throughput testing labs requiring low levels of organic leachables
  • Same design as MUP but a borosilicate glass vial reduces leachables that could come from plastics

Cellulose fibre - quantitative

  • Low ash filters in support gravimetric and other weight-based analyses
  • Available in a variety of thicknesses, retention sizes and surface treatments to support specific applications

Cellulose fibre - qualitative

  • High purity cotton filters support sample separation, clarification and purification in advance of analysis
  • Available in a variety of thicknesses, retention sizes and physical preparations to support specific applications

Glass and quartz fibre

  • The ability to capture very small particles supports environmental analysis and other applications requiring high retention rates
  • Available with a range of particle retention sizes, mechanical strengths and thermal stabilities to support specific applications


Key Products

Syringe Filters

  • Whatman Puradisc
  • Whatman GD/X
  • Whatman GD/XP
  • Whatman SPARTAN certified
  • Whatman Anotop
  • Whatman Mini-UniPrep (MUP) & G2
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Filter papers, and glass/quartz fibres

  • Cellulose fibre – quantitative
  • Cellulose fibre – qualitative
  • Glass and quartz fibre
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Resources

Resources will be available soon.

Products

Cytiva HyClone™ Fetal Bovine Serum, South American Origin

500ML FETAL BOVINE SERUM, Research GradeSouth American Origin, European Customers

Cytiva Whatman™ Binder-Free Glass Microfiber Filters GF/F Circles

Served as the basis filter for EPA standard for the toxicity characteristic leaching procedure. Whatman™ Binder-Free Glass Microfiber Filters GF/F Circles offers an extremely high loading capacity and flow rate. X25 TYPE GF/F 14.2CM/2GLASS FI

Cytiva PD SpinTrap™ G-25 Desalting Columns

Use for convenient and fast small-scale cleanup of proteins and other large biomolecules (>5000 Mr).  Cytiva Life Sciences™ PD SpinTrap™ G-25 Desalting Columns provide the option of running multiple samples in parallel. X50 PD SPINTRAP G-25

Cytiva Amersham™ Hyperfilm™ ECL

Use with all blue and green light chemiluminescent systems. Cytiva Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer. X50 Autoradiography film GE Healthcare Amersham

Cytiva Whatman™ UNIPLATE™ Microplates

Manufactured from polypropylene for superior chemical and thermal stability. Whatman™ UNIPLATE™ Microplates are suitable for simple filtrate collection and homogeneous assay techniques used in high-throughput screening (HTS). X25 UNIPLATE 5000 48 PP

Cytiva Cy3B Maleimides Mono-Reactive CyDye

Combines exceptional photostability with bright and intense signals. Cytiva Cy3B Maleimides Mono-Reactive CyDye is a more selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions. 1 MG CY3B MALEIMIDE

Cytiva HyClone™ RPMI 1640 Media

Designed to support cell culture growth and biomanufacturing processes. RPMI 1640 -GLUT 6X500ML

Cytiva Whatman™ Puradisc™ 4mm Nonsterile Syringe Filters

Used for HPLC samples containing low solid content and CE (Capillary Electrophoresis) samples X100 PURADISC 4 PVDF 0.45UM 4MM

HyClone products Cytiva 500 ML FETAL BOVINE SERUM, HEAT INACTIVATED,research grade, EU approved, South American origin

12350273 500 ML FETAL BOVINE SERUM, HEAT INACTIVATED,research grade, EU approved, South American origin 500 ML FETAL BOVINE SERUM, HEAT INACTIVATED,research grade, EU approved, South American origin

Cytiva Plastic TC Clamp

For use with the ÄKTA™ Pilot chromatography system. Cytiva Plastic TC Clamp is a white 25mm tri-clamp for providing a sanitary, secure connection. X4 CLAMP ATKAPILOT 25MM (PACK OF 4)

Cytiva PEEK Tubing

PEEK tubing comes in a variety of internal diameter sizes.  Cytiva Lifescience™ PEEK Tubing is an important chromatography accessory. TUBING PEEK 1/16 INCH, 0.75 MM I.D.

Cytiva Whatman™ Puradisc™ 4mm Sterile Syringe Filters

Designed for aqueous and organic solutions containing high debris levels. Whatman™ Puradisc DpPP Syringe Filters: Nonsterile tolerates high heat and mechanical stress. X50 PURADISC 4MM DEVICEST NYLON

Cytiva HiTrap™ SP Sepharose Fast Flow IEX Columns

Use for small-scale protein purifications Cytiva Life Sciences™ HiTrap™ SP Sepharose Fast Flow IEX Columns are strong cation exchanger columns for rapid separations either alone or connected in series. X5 HITRAP SP FAST FLOW 1ML

Cytiva Whatman™ Grade GB003 Blotting Paper

Ensures that no contamination will occur during the transfer steps. Whatman™ Grade GB003 Blotting Paper eliminates sheet to sheet variations with precisely cut membrane sizes. X100 BLOT PAPER GB003 46X57CM

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification. X10 HIS GRAVITRAP

Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. GLUTATHIONE SEPH 4 FF, 100 ML

Cytiva StrepTrap™ HP Prepacked Columns

Purify Strep(II)-tagged recombinant proteins by affinity chromatography. Cytiva Life Sciences™ StrepTrap™ HP Prepacked Columns are packed with a robust and stable Sepharose™ based medium which results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also high. StrepTrap HP 1 x 1mL

Cytiva Whatman™ HEPA-Vent Filter

Strengthened glass filter media by dual lamination with a tough polyester monofilament. Whatman™ HEPA-Vent Filters are ideal for air and gas filtration applications where high retention, dirt-holding capacity and flow rates are required. X10 HEPA-VENT GMF 50MM

Cytiva HiPrep™ 26/10 Desalting Columns

Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15mL per column. Cytiva HiPrep 26/10 Desalting Column is a convenient, easy-to-use column prepacked 53mL column with Sephadex G-25 Fine for desalting and buffer exchange. X4 Hiprep 26/10 Desalting column 53mL (pack of 4)

anti-Mouse IgG, peroxidase-linked species-specific whole antibody (from sheep) Secondary Antibody, Cytiva

Highly species-specific anti-Mouse IgG.  Cytiva Lifescience™ anti-Mouse IgG, peroxidase-linked species-specific whole antibody (from sheep) Secondary Antibody is designed for applications such as protein blotting, ELISA and immunocytochemistry. ECL MOUSE IGG, HRP-LINKED WHOLE AB (FROM SHEEP)(25800684) 00 Store in Fridge at 4C

Cytiva INV-907 Injection Kit

Range of manual and automated valves to meet variety of performance requirements. INJECTION KIT INV907

Cytiva Whatman™ Qualitative Filter Paper: Grade 1 Circles

Covers a wide range of laboratory applications and is frequently used for clarifying liquids. Whatman™ Qualitative Filter Paper: Grade 1 Circles are widely used standard grade filter papers for routine applications with medium retention and flow rates. X100 FILTER PAPER GR. 1 90MM

Cytiva Protein and Peptide Molecular Weight Markers

Run these markers to determine the molecular weight of uncharacterized protein samples with precision. Cytiva Life Sciences™ Protein and Peptide Molecular Weight Markers work by comparing unknowns with carefully selected standards. CALIBRATION KIT HIGH MOLECULAR WEIGHT (SDS) FORElectrophoresis (53-220KDa) 00Store in Fridge at

Cytiva UV Flow Cell

UV FLOW CELL, 2 MM

Cytiva Whatman™ 3MM Chr Chromatography Paper

Manufactured entirely from high-quality cotton linters with no additives and tested specifically for chromatographic techniques. Whatman™ Grade 3MM Chr Chromatography Paper ensures the wicking capability and uniformity of capillary action that are important in chemical separations. 3MM CHR BLOT ROLL 12.5CMX100M

Cytiva Inlet Filter Kit

Filters for buffer inlets as used with Inlet filter holder kit. Cytiva Inlet Filter Kit is a set of 5 polypropylene filters with 5 support screens. INLET FILTER SET, AKTA

Cytiva Borosilicate Glass Ethylene Propylene Complete Superloop

Use with aqueous solutions and alcohols.  Cytiva Lifescience™ Borosilicate Glass Ethylene Propylene Complete Superloop contains a springloaded valve made from titanium. SUPERLOOP 150ML M6 FITTING

Cytiva ETFE Ferrule

Ferrules used for chromatography tubing. Cytiva Lifescience™ ETFE Ferrule is made from ethylenetetra fluoroethylene and comes with different outside diameters. X10 FERRULE 3/16 INCH (PACK OF 10)

Cytiva Superdex™ 200 Prep Grade

Produce separation medium with high selectivity and resolution.  Cytiva Life Sciences™ Superdex™ 200 Prep Grade for purifying proteins of fractionation range: 10000 to 600000D SUPERDEX 200 PREP GRADE 150 ML

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Lab Recharge

Welcome to Cytiva Lab Recharge 2021

Offering solutions for all segments and applications in life sciences research including sample preparation,  protein purification, cell culture, filtration and Western blotting.

Contact your local Fisher Scientific Sales Representative and ask for your promo code to unlock your special prices.

Download your segment brochure now!

ÄKTA™ Quote/ Info Request
GEAkta

ÄKTA™ start protein purification system

You won’t believe how easy it is

From method creation and sample loading to fraction collection and data analysis, we’ve thought about how to make your work easier at every step. Let ÄKTA start transform your manual purification workflow into a convenient automated solution that delivers reproducible results

It’s like having an expert with you all the way.

UNICORN start system control software is user-friendly, guiding you through each step in your purification, from creating a protocol and setting up your run to evaluating your results and creating a custom report. See how UNICORN start software saves you time and gives you valuable insight into your run or purification.

ÄKTA start offers the following features:

  • Compact solution for quick and reliable one-step protein purification
  • Sample injection and fraction collection options
  • Quick start methods and templates for common purification techniques
  • Intuitive touchscreen display and real-time monitoring

Specifications:

  • Flow rate: 0.5-5mL/min
  • Pump type: peristaltic pump - single channel, four-roller pump head with low pulsation
  • Max. operating pressure: 0.5 Mpa
  • UV, fixed wavelength: Yes, 280 nm
  • Conductivity range: 0-300 mS/cm
  • Software: UNICORN start 1.0 or higher