Thermo Scientific Molecular Biology Solutions

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Sample Preparation
sample-prep

Sample Preparation

Nucleic acid isolation is a fundamental first step in most molecular biology applications, including cloning, PCR/qPCR, sequencing, in vitro transcription, transfection, northern and southern blotting. Thermo Scientific nucleic acid isolation products are optimized for high yield isolation of pure DNA or RNA from variety of sources.

Discover our column-based GeneJET kits, and magnetic bead-based MagJET kits for high-throughput automated or manual purification.

Sample Preparation Products

Catalog No. Description Uses Buy Now

Catalog No.

Description
GeneJET™ Plasmid Miniprep Kit
Uses
25 - 100 Preps
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Catalog No.

Description
GeneJET™ Plasmid Midiprep Kit
Uses
25 - 100 Preps
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Catalog No.

Description
GeneJET™ Plasmid Maxiprep Kit
Uses
10 - 25 Preps
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Catalog No.

Description
GeneJET™ Genomic DNA Purification Kit
Uses
50 - 250 Preps
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Catalog No.

Description
GeneJET™ RNA Purification Kit
Uses
50 - 250 Preps
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Catalog No.

Description
GeneJET™ PCR Purification Kit
Uses
50 - 250 Preps
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Catalog No.

Description
GeneJET™ Gel Extraction Kit
Uses
50 - 250 Preps
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Molecular Cloning
molecular-cloning

Molecular Cloning

Molecular cloning produces identical, recombinant DNA molecules for downstream applications, such as gene expression, protein purification and mutational analysis. Genes of interest are cloned into plasmid vectors, which are transformed into bacterial cells to produce bacterial clones. Thermo Scientific offers the most reliable genetic content, superior enzymes and advanced PCR tools to support and streamline your entire cloning workflow.

  • The Great Double Digestion Day

    Double Digestion in only 5 minutes? Thermo Scientific FastDigest Restriction Enzymes streamline the cloning process by providing complete digestion in as little as 5 minutes.

Find out more about Thermo Scientific cloning products starting from sample preparation to fragment amplification, DNA modification, electrophoresis and ligation.

Cloning Products

Catalog No. Description Uses Buy Now

Catalog No.

Description
Rapid DNA Ligation Kit
Uses
150 Reactions
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Catalog No.

Description
CloneJET™ PCR Cloning Kit
Uses
20 - 40 Reactions
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Catalog No.

Description
GeneJET™ Plasmid Miniprep
Uses
50 - 250 Preps
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Catalog No.

Description
T4 DNA Ligase
Uses
200 - 1000 U
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Catalog No.

Description
T4 Polynucleotide Kinase
Uses
500 - 2500 U
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PCR & qPCR
pcr-qpcr

PCR & qPCR

PCR is used to detect the presence or absence of specific DNA sequences, amplify large amounts of DNA for downstream use, or mutagenize coding DNA, for further genetic manipulation, mutational analysis, sequencing, genotyping or cloning. Depending on the polymerase used, amplification can be highly robust, quick or error proof, as in the case of high-fidelity enzymes. If starting material is a concern, direct PCR can be used to eliminate sample preparation altogether, or Reverse Transcription-PCR can be used if starting with mRNA. Quantitative real time PCR (qPCR) allows for real-time results with less variability due to sensitive fluorescent chemistry and elimination of post-PCR detection procedures.

Thermo Scientific offers PCR/qPCR solutions for your workflows; choose a polymerase or an appropriate PCR method for your needs using the following chart. Choose the best enzyme for your application or choose your detection chemistry with the PCR/qPCR Solutions brochure.

Master Mix Products

Catalog No. Description Uses Buy Now

Catalog No.

Description
Phusion™ Flash High-Fidelity PCR Master Mix
Uses
100 - 500 Reactions
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Catalog No.

Description
Fermentas™ Maxima™ SYBR™ Green/ROX qPCR Master Mix (2X)
Uses
2 - 10 Reactions
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DNA Polymerase

Catalog No. Description Uses Buy Now

Catalog No.

Description
Phusion™ Hot Start II High-Fidelity DNA Polymerase
Uses
100 - 500 U
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Description
Phire™ Hot Start II DNA Polymerase
Uses
200 - 1000 Reactions
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Description
Phire™ Green Hot Start II DNA Polymerase
Uses
200 - 1000 Reactions
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qPCR

Catalog No. Description Color Buy Now

Catalog No.

Description
Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless)
Color
HiGreen High ROX
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Catalog No.

Description
Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless)
Color
HiGreen Low ROX
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Catalog No.

Description
Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless)
Color
HiGreen Fluorescein
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Catalog No.

Description
Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless)
Color
HiGreen
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RT-PCR

Catalog No. Description Uses Buy Now

Catalog No.

Description
RevertAid™ First Strand cDNA Synthesis Kit
Uses
100 Reactions
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Catalog No.

Description
RevertAid™ Reverse Transcriptase
Uses
250 Reactions
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Catalog No.

Description
RevertAid™ H Minus First Strand cDNA Synthesis Kit
Uses
100 Reactions
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Catalog No.

Description
RevertAid™ H Minus Reverse Transcriptase
Uses
50 Reactions
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Catalog No.

Description
RiboLock RNase Inhibitor
Uses
2500 U
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