Estrazione e purificazione delle proteine

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophilic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.

SDS Solution, 20% Sodium Dodecyl Sulfate Solution, Molecular Biology/Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Formula molecolare: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Sinonimo: Sodium lauryl sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 IUPAC Name: sodium;dodecyl sulfate SMILES: CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]

Thermo Scientific™ Cocktail inibitori della proteasi Halt™

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Thermo Scientific™ Resina HisPur™ Ni-NTA

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

Thermo Scientific™ Reagenti di estrazione di proteine batteriche B-PER™

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

GE Healthcare Colonne preimpaccate Superdex™ 200 Increase 5/150 GL Tricorn™

Per cromatografia ad esclusione dimensionale e analisi di proteine con pesi molecolare tra 10,000 e 600,000. Le colonne preimpaccate  GE Healthcare Lifescience™ Superdex™ 200 Increase 5/150 GL Tricorn™ sono progettate per purificazione preparativa su piccola scala.

Invitrogen™ Molecular Probes™ EZQ™ Protein Quantitation Kit

Fluorescence-based protein assay facilitates fast quantitation of protein samples prepared for gel electrophoresis

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

A highly sensitive endpoint assay that accurately measures and detects endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acids or antibody sample using the Amebocyte Lysate Assay.

Corning™ Concentratori Spin-X™ UF

Disposable, single use only ultrafiltration devices with polyethersulfone membranes (PES) for the centrifugal concentration and/or purification of biological fluids and aqueous solutions

Thermo Scientific™ anti-HA Epitope Tag Clone: 2-2.2.14

HA Epitope Tag Monoclonal antibody 26181 from Thermo Scientific™ specifically detects HA Epitope Tag in Tag samples, and it is validated for Immunoprecipitation

Thermo Scientific™ Colonne in plastica monouso Pierce™

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques.

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds.

Thermo Scientific™ Tampone RIPA Pierce™

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.

GE Healthcare Superdex™ 75 Prep Grade

Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins

Thermo Scientific™ Tampone di lisaggio IP Pierce™

Maximize cell lysate yield, purity and compatibility with this immunoprecipitation-optimized protein extraction reagent.

Thermo Scientific™ Biglie magnetiche NHS-attivate Pierce™

Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups.

Thermo Scientific™ Kit di frazionamento subcellulare di proteine per cellule in coltura

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments

Thermo Scientific™ Colonne di centrifugazione Pierce™

Pack these empty 0.5 to 10mL spin columns with agarose beads to perform small-scale affinity purification methods in microcentrifuge, 15mL conical or 50mL conical tubes.

Thermo Scientific™ Colonne di microcentrifugazione per rimozione detergenti Pierce™ e piastre

Rapidly eliminate high concentrations of detergents with minimal sample loss with our spin column or filter-plate formats for centrifuge processing.

Thermo Scientific™ Kit di estrazione nucleare e citoplasmatica NE-PER™

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Thermo Scientific™ Soluzione detergente NP-40 Surfact-Amps™

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution in 10mL glass ampules.

Invitrogen™ DYNAL™ DynaMag™ 50 Magnet

Optimized for efficient cell isolation using Dynabeads™

Merck™ BugBuster™ Protein Extraction Reagent

Reagent for protein sample preparation

GE Healthcare Colonne preimpaccate HisTrap™ HP

Per la purificazione diretta di proteine marcate con istidina da cellule non chiarificate omogeneizzate. Le colonne GE Healthcare Life Sciences™ HisTrap™ HP sono preimpaccate con Ni Sepharose™ High Performance e consentono la facile purificazione in un solo passaggio di proteine marcate con istidina tramite cromatografia di affinità con ioni metallici immobilizzati (IMAC).

GE Healthcare Streptavidin HP MultiTrap™ Plates

Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates

Sartorius™ Vivapure™ LentiSELECT 500 Purification and Concentration Kit

Fast purification of up to 3 x109 viral particles

GE Healthcare Proteasi trombina

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

  spinner