Western Blotting Reagents, Membranes, Supplies

GE Healthcare Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  GE Healthcare Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. GE Healthcare Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

GE Healthcare Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. GE Healthcare Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

GE Healthcare Amersham™ Hybond™ PVDF Membranes: Rolls

Ideal for Western blotting applications where stripping and reprobing are necessary.  GE Healthcare Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls  are hydrophobic PVDF membranes.

Invitrogen™ XCell SureLock™ Mini-Cell and XCell II™ Blot Module

Quickly and easily run and blot Novex minigels

Invitrogen™ Mini Blot Module

Wet transfer device used exclusively with the Bolt Minigel Tank

MP Biomedicals™ BCIP/NBT Liq. Substrate Plus, 500mL

Stabilized chromogen solution with greater sensitivity and produces little or no background staining compared with other alternatives.

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Thermo Scientific™ Western Blot Roller

A simple, reusable tool for gently and easily removing trapped air bubbles when assembling transfer stacks in semi-dry or wet-tank transfer cassettes.

Invitrogen™ Mini Gel Tank and Blot Module Set

Provides all of the necessary devices for protein separation and Western blotting using Novex™ gels, NuPAGE™ gels, and Bolt™ Bis-Tris Plus gels

Thermo Scientific™ Blocker™ Casein

Block membranes, plates and other samples with these ready-to-use PBS or TBS solutions of purified casein for Western blot, ELISA and other detection methods.

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

GE Healthcare Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls

Use for robust protein blotting. GE Healthcare Life Sciences™ Amersham™ Protran™ Supported NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Invitrogen™ iBlot™ Transfer Stack, PVDF, mini

Used to transfer proteins with the iBlot Gel Transfer Device

Invitrogen™ Bolt™ Welcome Pack + iBlot™ 2 System

A complete protein separation-to-western blot solution by combining a Bolt Welcome Pack A with the iBlot 2 Gel Transfer Device and iBlot 2 Transfer Stack (nitrocellulose, regular size) in lieu of one box of Bolt gels

Thermo Scientific™ CL-XPosure™ Film, 7 x 9.5 in. (18 x 24 cm)

Capture results of ECL-based Western blots and other chemiluminescent protein or nucleic acid assays using this economically priced, clear-blue X-ray film.

IBA Lifesciences™ Biotin Blocking Buffer

Biotin Blocking Buffer is recommended for blocking biotinylated proteins on Western blots prior to detection of Strep-tag proteins with the Strep-Tactin AP conjugate or Strep-Tactin HRP conjugate.

Invitrogen™ H2DCFDA (H2-DCF, DCF)

Chemically reduced form of fluorescein used as an indicator for reactive oxygen species in cells

Thermo Scientific™ HisProbe™-HRP Conjugate

Directly detect histidine-tagged fusion proteins and other histidine-rich proteins with this nickel-activated horseradish peroxidase (HRP) derivative.

Thermo Scientific™ Western Blotting Filter Papers, 0.83-mm Thick, 8 x 13.5 cm

Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.

Invitrogen™ XCell II™ Blot Module

Easy transfer of proteins or nucleic acids from mini-gels to membranes

Merck Millipore Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

GE Healthcare Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. GE Healthcare Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Miser™ Antibody Extender Solution NC

Enable Western blot detection with much less primary antibody by treating nitrocellulose membrane with this reagent.

Thermo Scientific™ Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Mouse

For mid-to-low femtogram-level detection and Western blot development less than 1 hour after gel transfer.

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Streptavidin Protein, AP

Streptavidin Protein, AP conjugate, 21324, from Invitrogen™, 21324, from Invitrogen™ Species Reactivity: ; Applications: ELISA, Western Blot.

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