Cytiva

Cytiva Cytiva

Cytiva: speed, efficiency, capacity

Cytiva is a global provider of technologies and services that help advance and accelerate the development and manufacture of therapeutics. With a heritage of innovation, we continue to move forward, building on the brands you know and have come to rely on for quality and reliability. Our diverse portfolio includes well-recognised brands such as ÄKTA™, Amersham™, HyClone™, MabSelect™ and Whatman™. Cytiva brings speed, efficiency and capacity to life sciences research and manufacturing, enabling the delivery of transformative medicines to patients.

Protein Research

Choose from Cytiva's portfolio of protein purification tools, from Sephadex™, the chromatography resin that revolutionised protein purification, to the newest magnetic bead-based technology. Cytiva's products support a broad range of chromatography resins and resin methods, including affinity purification, gel filtration, desalting, ion exchange and hydrophobic interaction, all in formats to meet your application requirements.

  • Loose resin up to 500g
  • Chromatography columns such as HiTrap™, SpinTrap™, GraviTrap™ and Tricorn™ columns
  • Pre-packed columns for convenience and efficiency

Western Blotting

Amersham™ Western blotting products deliver reliable protein identification for you time after time: meaningful data, reproducible results.


Key Products

Cell Culture

HyClone™ serum, media buffers and process liquids have helped advance drug discovery and bioproduction processes since 1967, and continue to deliver quality and consistency.

HyClone™ Classical and Serum-Free Media »

Culture cells using our complete line of HyClone™ classical, speciality and transfection media products that support cell growth of CHO, SF9, HEK293, hybridoma, myeloma, insect, NS0 and viral vaccine cell lines.

HyClone™ Fetal Bovine Serum (FBS), Calf Serum, Fetal Bovine Serum Alternatives, and Supplements »

Enhance cell culture performance using a variety of HyClone™ supplements, including Fetal Bovine Serum (FBS), calf serum, engineered serum (FetalClone™ 1, 2, 3) and serum-free supplements (HyClone™ Cell Boost 1-6).

HyClone™ Antibiotics and Reagents »

Separate attachment-dependent cells from substrate or reduce the effect of bacteria, mould or yeast contaminants in your cell culture process.

HyClone™ Stem Cell Products »

Grow stem cells with ease using our specialty line of stem cell products tailored to optimise cell culture performance for a variety of stem cells, including induced pluripotent stem cells (iPSC), mesenchymal stem cells (mSC), human embryonic stem cells (hESC) and epithelial stem cells.

HyClone™ Buffers and Process Liquids »

Manage, control and regulate pH, temperature, osmotic pressure, carbon dioxide and oxygen for your specific cell culture.

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Key Products

DNA and RNA Research

When nucleic acid (DNA or RNA) sample is scarce, amplification can be used to expand the sample quantity for use in multiple experiments. Selecting the most suitable amplification technology will depend on sample type, the molecular biology application where it will be used, and the need to preserve sample for additional testing. Some common technologies for DNA amplification are Phi29 polymerase technology, polymerase chain reaction (PCR), rolling circle amplification (RCA), and isothermal multiple strand displacement amplification (MDA).

Phi29 polymerase technology

Phi29 DNA polymerase-based isothermal DNA amplification is a simple, reliable alternative to other DNA amplification procedures such as polymerase chain reaction (PCR). The highly processive Phi29 DNA polymerase elicits strong strand displacement enabling rapid DNA replication from multiple sites. 

Whole genome amplification

GenomiPhi™ products, powered by Phi29 polymerase technology, are available in kits that provide an easy-to-use method which delivers highly representative and reliable whole genome amplification. Each kit contains all the components necessary for whole-genome amplification by utilising isothermal multiple strand displacement amplification, with minimal hands-on time. Compared with conventional PCR amplification techniques, MDA generates larger sized products with a lower error frequency.

Rolling circle amplification

TempliPhi™, based on Phi29 DNA polymerase, is used to exponentially amplify single- or double-stranded circular DNA by rolling circle amplification (RCA). This isothermal amplification produces microgram quantities of DNA from picograms of starting material in 4 to 6 hours.


Whatman™ Filtration

Filtration is an indispensable step in sample preparation and many analytical methods. Cytiva's Whatman™ products provide the quality, consistency, reliability and convenience to help your lab run smoothly. With a large variety of available sizes, types and specifications, you can be sure to find a filter that fits your application, equipment and workflow.

  • Cellulose, glass and quartz fibre filters
  • Membrane filters
  • Syringe and syringeless filtration devices
  • Capsule filters
  • Sterile and non-sterile options
  • Lab essentials

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Whatman Puradisc syringe filter

  • High performance, dependable single membrane layer filter
  • Available in 11 different membrane types and seven pore sizes to work for samples of many chemical compositions and volumes

Whatman GD/X™ syringe filter

  • Used for heavy, thick, or viscous hard-to-filter samples
  • Multi-gradient glass-fibre layer pre-filters large particles which increases flow rate and volume through the main membrane

Whatman GD/XP syringe filter

  • Used for heavy, thick, or viscous samples with low protein binding and low metals extractables
  • Polypropylene pre-filtration stack provides increased flow rate and volume flow through while eliminating protein binding and inorganic ion extractables that could come from glass fibre pre-filtration 

Whatman SPARTAN™ certified

  • Every batch is tested and certified for HPLC use to ensure batch-to-batch consistency and low levels of UV-absorbing substances
  • Regenerated cellulose membrane exhibits very low protein binding and low levels of extractables in water, methanol and acetonitrile

Whatman Anotop™

  • Recommended for use in ion chromatography 
  • Alumina-based Anopore membrane has very low ion extractables and highly uniform capillary pore structure

Whatman Mini-UniPrep™ (MUP)

  • Designed for HPLC and UHPLC autosamplers to support high throughput testing labs
  • Combines syringe, syringe filter, autosampler vial, cap and septum in a single device for increased efficiency in consumable management

Whatman Mini-UniPrep™ (MUP) G2

  • Designed for HPLC and UHPLC autosamplers to support high throughput testing labs requiring low levels of organic leachables
  • Same design as MUP but a borosilicate glass vial reduces leachables that could come from plastics

Cellulose fibre - quantitative

  • Low ash filters in support gravimetric and other weight-based analyses
  • Available in a variety of thicknesses, retention sizes and surface treatments to support specific applications

Cellulose fibre - qualitative

  • High purity cotton filters support sample separation, clarification and purification in advance of analysis
  • Available in a variety of thicknesses, retention sizes and physical preparations to support specific applications

Glass and quartz fibre

  • The ability to capture very small particles supports environmental analysis and other applications requiring high retention rates
  • Available with a range of particle retention sizes, mechanical strengths and thermal stabilities to support specific applications


Key Products

Syringe Filters

  • Whatman Puradisc
  • Whatman GD/X
  • Whatman GD/XP
  • Whatman SPARTAN certified
  • Whatman Anotop
  • Whatman Mini-UniPrep (MUP) and G2
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Filter papers, and glass/quartz fibres

  • Cellulose fibre – quantitative
  • Cellulose fibre – qualitative
  • Glass and quartz fibre
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Resources

Resources will be available soon.

Products

Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity. ECL Prime Western Blotting Detection Reagent for 3000 cm2 membrane

Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background. X1 Amersham Protran 0.1um Nitrocellulose 300mm x 4m roll

Cytiva Column Holder

Holds ÅKTA columns. Cytiva Column Holder for columns with outer diameter between 10 and 50mm. COLUMN HOLDER

Cytiva HiLoad™ Superdex™ 75 pg

For high resolution preparative gel filtration chromatography with short run times and good recovery.  Cytiva Lifescience™ HiLoad™ Superdex™ 75 pg are prepacked XK columns. HILOAD 16/600 SUPERDEX 75 PG

Cytiva Amersham™ Hybond™ PVDF Membranes: Rolls

Ideal for Western blotting applications where stripping and reprobing are necessary.  Cytiva Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls  are hydrophobic PVDF membranes. X1 Amersham Hybond LFP 0.2um PVDF 254mm x 4m roll

Cytiva Disposable PD-10 Desalting Columns

Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery. X30 Disposable PD-10 desalting column

Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. X10 Amersham Hybond 0.45um PVDF 100mm x 100mm sheets

Cytiva Illustra ExoProStar™ 1-Step

Enzymatic PCR clean up technology with illustra Exonuclease I and Alkaline Phosphatase combined in one tube to remove unincorporated primers and dNTPs. Cytiva illustra ExoProStar™ 1-Step comes in one tube for maximum convenience. ILLUSTRA EXOPROSTAR-1 STEP (20 RCN)

Cytiva Poly(I)-Poly(C)

For stimulation of interferon production in vitro.  Cytiva Poly(I)-Poly(C) is supplied as a sterile lyophilised powder. 265 MG POLY (I)-POLY(C) STERILE 265MG STORE AT-20°C Store in Freezer at -20C

Cytiva HyClone™ Phosphate Buffered Saline (PBS)

Buffer cell culture growth with PBS 1000ML PBS 1X 0.0067M PO4

Cytiva Whatman™ 19mm Nuclepore™ Polycarbonate Track-Etched Membranes

Manufactured from high-quality polycarbonate film and track-etched for high pore size precision. Whatman™ 19mm Nuclepore™ Polycarbonate Hydrophilic Membranes combine high flow rates and chemical and thermal resistance with sharply defined pores, making them suitable for microscopy, cytology, parasitology, and certain air and oceanography analyses. X100 PC MB 19MM 1.0UM

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent  provides highly intense signal output, for detection of medium to very low protein levels. WB DETECTION REAGENT KIT, ECL SELECT, HIGH SENSITIvity chemiluminescent substrate for1000cm2 of

Cytiva Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media

USe for  separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins.  Cytiva Life Sciences™ Heparin Sepharose™ 6 Fast Flow Affinity Chromatography Media is fast and reliable and highly suitable for process-scale purification processes. 50 ML HEPARIN SEPHAROSE 6 FAST FLOW 50ML STORE ATambient Store at Room Temperature

Cytiva Whatman™ Benchkote Plus Surface Protector Sheets

Prevent many common sources of laboratory contamination, such as those involving hazardous materials on shared workspace benches. Whatman™ Benchkote Plus bench liner can also help with the disposal of hazardous materials. X50 SHEET BENCHKOTE+ 50X60 CM

Cytiva Whatman™ Binder-Free Glass Microfiber Filters, Grade GF/B

Filters fine particles with high wet-strength and high loading capacity. Whatman™ Binder-Free Microfiber Filters, Grade GF/B are particularly useful for liquid clarification and liquid scintillation counting. X25 FILTER GLASS CIRCLE GF/B 185MM

Cytiva HiTrap™ Protein G HP Prepacked Columns

Use for fast, easy separations of IgG from different species and subclasses. Cytiva Life Sciences™ HiTrap™ Protein G HP Prepacked Columns are prepacked with Protein G Sepharose™. X5 HITrap Protein G High performance 5mL Store in

Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species. SEPHADEX LH-20 500 GRS

Cytiva Accessories for Multiphor™ II Electrophoresis System

X500 Electrode wicks GE Healthcare MultiPhor

Cytiva HyClone™ DMEM/F12 1:1 Media

Used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. HyClone™ DMEM/F12 1:1 Media supports cell culture growth and biomanufacturing processes and is manufactured using ISO 9001:2000-certified processes. DMEM F12 1:1 +GLUT 500ML

Cytiva illustra™ GenomiPhi V2 DNA Amplification Kit

Amplify whole genomes with high efficiency and representation. Cytiva Life Sciences™ illustra™ GenomiPhi V2 DNA Amplification Kit provides whole genome amplification with yields of 4 to 7μg. ILLUSTRA GENOMIPHI V2 DNA AMPLIFICATION KIT 25reactions25reactions Store in freezer at -80C

Cytiva HyClone™ SFM4Insect™ Media

Support growth of many key insect cell lines and production of a variety of recombinant proteins. HyClone™ SFM4Insect™ Media is free of animal-derived components (ADCF). SFM4INSECT + L-GLUTAMINE LIQUID 1LL-Glutamine Liquid, Size=1000 mL

Cytiva GSTrap™ 4B Prepacked Columns: 7 × 25mm

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 × 25mm are prepacked with Glutathione Sepharose™ 4B GSTRAP 4B, 1 X 1 ML

Cytiva Whatman™ Puradisc™ 4mm Sterile Syringe Filters

Designed for aqueous and organic solutions containing high debris levels. Whatman™ Puradisc DpPP Syringe Filters: Nonsterile tolerates high heat and mechanical stress. X50 SYRINGE FILTERS 4MM PVDF, STERILE 0.2 MICRONPORE SIZE

Cytiva Whatman™ Qualitative Filter Paper: Grade 1 Sheets

Covers a wide range of laboratory applications and is frequently used for clarifying liquids. Whatman™ Qualitative Filter Paper: Grade 1 Sheets are widely used standard grade filter paper for routine applications with medium retention and flow rates. X100 TYPE 1 60X60CM 100/PKFILTER S

Cytiva HyClone™ McCoy's 5A Media

Support cell culture growth and biomanufacturing processes with HyClone™ McCoy's 5A Media, manufactured using ISO 9001-certified processes.

McCoy's 5A Medium w L-Glutamine/Phenol red,6x500ml

Cytiva Whatman™ Qualitative Filter Paper: Grade 4 Circles

Filters and retains course particles for monitoring air pollution. Whatman™ Qualitative Filter Paper: Grade 4 Circles are used when high flow rates in air pollution monitoring are required and the collection of fine particles is not critical. X100 FILTER PAPERS, NO.4 DIAMETER 18.5CM(1004 185) (PACK OF 100)

Cytiva Rainbow™ Molecular Weight Markers

Monitor progress of protein electrophoresis and assess transfer efficiency and molecular weight of blotted proteins without staining. Cytiva Life Sciences™ Rainbow™ Molecular Weight Markers use bright, distinctive colors to allow easier confirmation of transfer to blotting membranes and orientation. FULL RNG MOL WT MARKER RAINBOW

Cytiva Whatman™ Lens Cleaning Tissue

Provides scratch-free cleaning solutions. Whatman™ Lens Cleaning Tissue safely removes surface moisture and grease. X100 TISSUES LENS CLEANING 105 300X200MM(2105 862)(PACK OF 100)

Cytiva Polyethylene (PE) Capillary Tubing

For use with chromatography applications CAPILLARY TUB PTFE,5M,1.1ID,1.8OD

Cytiva pI Markers

Run these markers in parallel with the experimental samples on isoelectric focusing gels. Cytiva Life Sciences™ pI Markers are lyophilized mixtures of stable, salt-free, highly purified proteins. IEF MARKER, HIGH PI CALIBRATION KIT PH 5 TO 10.50 Store in Fridge at 4C

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Lab Recharge

Welcome to Cytiva Lab Recharge 2021

Offering solutions for all segments and applications in life sciences research including sample preparation,  protein purification, cell culture, filtration and Western blotting.

Contact your local Fisher Scientific Sales Representative and ask for your promo code to unlock your special prices.

Download your segment brochure now!

ÄKTA™ Quote/ Info Request
GEAkta

ÄKTA™ start protein purification system

You won’t believe how easy it is.

From method creation and sample loading to fraction collection and data analysis, we’ve thought about how to make your work easier at every step. Let ÄKTA start transform your manual purification workflow into a convenient automated solution that delivers reproducible results.

It’s like having an expert with you all the way.

UNICORN start system control software is user-friendly, guiding you through each step in your purification, from creating a protocol and setting up your run to evaluating your results and creating a custom report. See how UNICORN start software saves you time and gives you valuable insight into your run or purification.

ÄKTA start offers the following features:

  • Compact solution for quick and reliable one-step protein purification
  • Sample injection and fraction collection options
  • Quick start methods and templates for common purification techniques
  • Intuitive touchscreen display and real-time monitoring

Specifications:

  • Flow rate: 0.5 to 5mL/min
  • Pump type: peristaltic pump - single channel, four-roller pump head with low pulsation
  • Max. operating pressure: 0.5MPa
  • UV, fixed wavelength: Yes, 280nm
  • Conductivity range: 0 to 300mS/cm
  • Software: UNICORN start 1.0 or higher