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BD Caspase-3 Enzyme Set
Gene that is required for normal apoptosis during development
Marque: BD 556473
Includes: Recombinant Human Active Caspase-3: 5μg, Ac-DEVD-AFC, Caspase-3 Fluorogenic Substrate: 1.0mg, Ac-DEVD-CHO, Caspase-3 Inhibitor: 1.0mg
Description
Caspases are cysteine proteases that play central role in apoptosis. The caspase family was discovered by searching human cDNA libraries for sequences homologous to ced-3, a C. The rate of caspase-3 enzymatic hydrolysis can be measured by release of AFC, from Ac-DEVD-AFC caspase substrate, through emission at 480-520nm (peak at 505nm) upon excitation at 400nm using UV spectrofluorometry
- Consisting of 12kDa and 17kDa subunit derived from 32kDa pro-enzyme (pro- caspase-3)
- Cleaves PARP, an enzyme that is involved in DNA repair and genomic maintenance
- The cleavage site in PARP is C-terminal to Asp-216
- Cleaves the tetrapeptide between D and AFC, thus releasing the fluorogenic AFC
- Expressed in E.coli and purified
Enzyme assay
Spécification
| Enzyme Assay | |
| 20 Tests | |
| Human |
| Caspase-3 Enzyme Set | |
| -80°C |
Research use only