Thermo Scientific Molecular Biology Solutions
Sample Preparation
Nucleic acid isolation is a fundamental first step in most molecular biology applications, including cloning, PCR/qPCR, sequencing, in vitro transcription, transfection, northern and southern blotting. Thermo Scientific nucleic acid isolation products are optimized for high yield isolation of pure DNA or RNA from variety of sources.
Discover our column-based GeneJET kits, and magnetic bead-based MagJET kits for high-throughput automated or manual purification.
Sample Preparation Products
| Catalog No. | Description | Uses | Buy Now |
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GeneJET™ Plasmid Miniprep Kit |
25 - 100 Preps |
Purchase Now | |
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GeneJET™ Plasmid Midiprep Kit |
25 - 100 Preps |
Purchase Now | |
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GeneJET™ Plasmid Maxiprep Kit |
10 - 25 Preps |
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GeneJET™ Genomic DNA Purification Kit |
50 - 250 Preps |
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GeneJET™ RNA Purification Kit |
50 - 250 Preps |
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GeneJET™ PCR Purification Kit |
50 - 250 Preps |
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GeneJET™ Gel Extraction Kit |
50 - 250 Preps |
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Molecular Cloning
Molecular cloning produces identical, recombinant DNA molecules for downstream applications, such as gene expression, protein purification and mutational analysis. Genes of interest are cloned into plasmid vectors, which are transformed into bacterial cells to produce bacterial clones. Thermo Scientific offers the most reliable genetic content, superior enzymes and advanced PCR tools to support and streamline your entire cloning workflow.
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The Great Double Digestion Day
Double Digestion in only 5 minutes? Thermo Scientific FastDigest Restriction Enzymes streamline the cloning process by providing complete digestion in as little as 5 minutes.
Find out more about Thermo Scientific cloning products starting from sample preparation to fragment amplification, DNA modification, electrophoresis and ligation.
Cloning Products
| Catalog No. | Description | Uses | Buy Now |
|---|---|---|---|
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Rapid DNA Ligation Kit |
150 Reactions |
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CloneJET™ PCR Cloning Kit |
20 - 40 Reactions |
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GeneJET™ Plasmid Miniprep |
50 - 250 Preps |
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T4 DNA Ligase |
200 - 1000 U |
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T4 Polynucleotide Kinase |
500 - 2500 U |
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PCR & qPCR
PCR is used to detect the presence or absence of specific DNA sequences, amplify large amounts of DNA for downstream use, or mutagenize coding DNA, for further genetic manipulation, mutational analysis, sequencing, genotyping or cloning. Depending on the polymerase used, amplification can be highly robust, quick or error proof, as in the case of high-fidelity enzymes. If starting material is a concern, direct PCR can be used to eliminate sample preparation altogether, or Reverse Transcription-PCR can be used if starting with mRNA. Quantitative real time PCR (qPCR) allows for real-time results with less variability due to sensitive fluorescent chemistry and elimination of post-PCR detection procedures.
Thermo Scientific offers PCR/qPCR solutions for your workflows; choose a polymerase or an appropriate PCR method for your needs using the following chart. Choose the best enzyme for your application or choose your detection chemistry with the PCR/qPCR Solutions brochure.
Master Mix Products
| Catalog No. | Description | Uses | Buy Now |
|---|---|---|---|
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Phusion™ Flash High-Fidelity PCR Master Mix |
100 - 500 Reactions |
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Fermentas™ Maxima™ SYBR™ Green/ROX qPCR Master Mix (2X) |
2 - 10 Reactions |
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DNA Polymerase
| Catalog No. | Description | Uses | Buy Now |
|---|---|---|---|
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Phusion™ Hot Start II High-Fidelity DNA Polymerase |
100 - 500 U |
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Phire™ Hot Start II DNA Polymerase |
200 - 1000 Reactions |
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Phire™ Green Hot Start II DNA Polymerase |
200 - 1000 Reactions |
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qPCR
| Catalog No. | Description | Color | Buy Now |
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Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless) |
HiGreen High ROX |
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Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless) |
HiGreen Low ROX |
Purchase Now | |
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Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless) |
HiGreen Fluorescein |
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Luminaris™ Color HiGreen qPCR Master Mixes and Luminaris™ HiGreen qPCR Master Mixes (colorless) |
HiGreen |
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RT-PCR
| Catalog No. | Description | Uses | Buy Now |
|---|---|---|---|
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RevertAid™ First Strand cDNA Synthesis Kit |
100 Reactions |
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RevertAid™ Reverse Transcriptase |
250 Reactions |
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RevertAid™ H Minus First Strand cDNA Synthesis Kit |
100 Reactions |
Purchase Now | |
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RevertAid™ H Minus Reverse Transcriptase |
50 Reactions |
Purchase Now | |
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RiboLock RNase Inhibitor |
2500 U |
Purchase Now |
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Thermo Scientific Molecular Biology Tools Brochure
Powerful Thermo Scientific Molecular Biology Tools support and facilitate your restriction digestion, cloning, nucleic acid purification, electrophoresis and nucleotide workflows.
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Thermo Scientific Solutions for Cloning Guide
Shop tools, reagents, thermal cyclers and consumables; even pre-made, fully sequenced clones.
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Thermo Scientific GeneJET Nucleic Acid Purification Kits Brochure
The Thermo Scientific GeneJET product line contains essential nucleic acid purification tools to support and facilitate your experimental workflow.
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Thermo Scientific PCR Reagents Selection Guide
Compare applications, restrictions and performance for four different PCR workflows. Find kits and products.
More
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Thermo Scientific FastDigest Restriction Enzymes Brochure
Thermo Scientific FastDigest enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Thermo Scientific aLICator Cloning System Workflow
Achieve successful cloning robust protein expression with Thermo Scientific's aLICator system.
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Thermo Scientific Phusion and Phire DNA Polymerases Brochure
Thermo Scientific offers Phusion and Phire: accurate, fast and powerful Polymerases for better PCR.
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Thermo Scientific MagJET Kits Workflow
Thermo Scientific MagJET nucleic acid purification kits utilize magnetic bead-based technology, allowing for highly efficient nucleic acid isolation.
- Thermo Scientific MagJET Kits Workflow (217.7 KB)
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Thermo Scientific PCR and qPCR Solutions Guide
Thermo Scientific multiple polymerases and master mixes deliver top performance over a range of applications.