PCR and qPCR
Thermo Scientific™ Phusion High-Fidelity DNA Polymerase
Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available. 100 RXN PHUSION HIGH-FIDELITY PCR MASTER MIX GCbuffer formulation (2 x 1.25mL), 2X Master Mix
Applied Biosystems™ TaqMan™ High-Capacity RNA-to-cDNA Kit
Designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR™ Green PCR Master Mix, and other PCR enzymes 50 RXN HIGH CAPACITY RNA-TO-CDNA KIT 50REACTIONSStore at -20 C
Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), No AmpErase™ UNG
TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR. 1250 RXN FAST UNIVERSAL PCR MASTER MIX (2X), NO
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
Optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. 200RXN SuperScript IV First-Strand Synthesis System
Invitrogen™ Nuclease-Free Water (not DEPC-Treated)
NUCLEASE-FREE WATER 5 X 100 MLAmbion® Nuclease-free water has not been treated
Applied Biosystems™ MicroAmp™ Fast Optical 96-Well Reaction Plate, 0.1mL
Reduces PCR reaction time from 2 hours to as little as 25 minutes X200 MICROAMP, FAST OPTICAL 96-WELL REACTIONplate with barcode, 0.1mL
Invitrogen™ SuperScript™ IV Reverse Transcriptase
A proprietary MMLV mutant with superior robustness and reliability in RT reactions. 40000UNIT SuperScript IV Reverse Transcriptase
Fisherbrand™ 96-Well Low-Profile, Skirted PCR Plates, Natural
Fit most thermal cyclers X25 Fisherbrand PCR plate 96-wells, full skirt, PP, red - FB-0800/R
Invitrogen™ Oligo(dT) 20 Primer
Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C OLIGO DT(20) PRIMER
Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA KIT, HIGH CAPACITY CDNA RT1000 reactions
Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate with Barcode
Optimized to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification MicroAmp(R) Optical 96-well Reaction PlatewithBarcode (code 128), 25-Pack, 500/Pkg
SuperScript™, Platinum™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. SSIII 1-STEP QRT-PCR 500500 reactions
PowerUp™, SYBR™ PowerUp™ SYBR™ Green Master Mix
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. X10 POWERUP SYBR MASTER MIX,10X1ML
Applied Biosystems™ MicroAmp™ Optical 96-Well Reaction Plate
Designed to provide unmatched temperature accuracy and uniformity for fast, efficient PCR amplification X500 MICROAMP OPTICAL 96-WELL REACTION PLATE
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
Nucleaic acid purfication PHENOL/CHLOROFORM/ALCOHOL
Invitrogen™ MultiScribe™ Reverse Transcriptase
A recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase that has been optimized for TaqMan-based assays 100 UL MULTISCRIBE REVERSE TRANSCRIPTASE 100µLStore at -20 C
Invitrogen™ EDTA (0.5M), pH 8.0
Ready-to-use, certified RNase-free 500 ML 0.5 M EDTA, PH 8.0 500ML STORE AT ROOMtemperature
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
For use with reverse transcriptase-PCR (RT-PCR) 2000 RXN REVERSE TRANSCRIPTION REAGENTS (10 PACK)
Invitrogen™ SuperScript™ III Reverse Transcriptase
Proprietary mutant of SuperScript II RT 2 KU REVERSE TRANSCRIPTASE SUPERSCRIPT(TM) IIIStore at -20C
Applied Biosystems™ TaqMan™ Fast Advanced Master Mix
TaqMan™ Fast Advanced Master Mix has been designed to match or exceed the performance of standard master mixes, delivering shorter run times (<40 minutes). TAQMAN FAST ADVANCED MASTER MIX (1 X 50 ML)
Thermo Scientific™ ABgene™ Thermo-Fast™ 96-Well Semi-Skirted PCR Plates
Patented segmented design allows plate to be cut into 24- and 48-well sections X25 THERMOFAST SSK 96X0,2ML GREEN(VE=25Stck.)
Applied Biosystems™ Random Hexamers (50mM)
Serve as primers for DNA synthesis by a DNA polymerase or reverse transcriptase 1 SET Random Hexamers (50um) 1kit Store at -20 C
Thermo Scientific™ Thermo-Fast™ 96-Well Full-Skirted Plates
96-well full-skirted PCR automation compatible plate for use in PCR and qPCR applications. X25 THERMOFAST SK 96X0,2ML WHITEwhite (VE=25Stck.)
Invitrogen™ UltraPure™ Buffer-Saturated Phenol
Buffer for nucleic acid purification BUFFER SATURATED PHENOL
Applied Biosystems™ TaqMan™ Universal Master Mix II, With UNG
TaqMan™ Universal Master Mix II is a convenient mix of components (except primers, probes, template & water) necessary to perform a real-time polymerase chain reaction (PCR). X5 TAQMAN UNIVERSAL MASTER MIX II, WITH UNG 5
SYBR™ Fast SYBR™ Green Master Mix
Designed for speed, provides a fast, reliable and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range or PCR efficiency. 5000 RXN FAST SYBR GREEN MASTER MIX BULK PACK (1x 50mL) 5000reactions Store at -20 C
Applied Biosystems™ MicroAmp™ Optical 8-Cap Strips
Tight seal minimizes evaporation and contamination X300 MICROAMP OPTICAL 8-CAP STRIP STORE AT ROOMtemperature
Applied Biosystems™ MicroAmp™ 96-Well Support Base
Designed to hold MicroAmp Reaction Tubes in Tray/Retainer Sets or MicroAmp 48- and 96-Well Plates for convenient use in pre- and post-PCR sample processing X10 MICROAMP 96-WELL SUPPORT BASE STORE AT ROOMtemperature
Thermo Scientific™ 0.5 mL Individual Tubes
Minimize sample loss with the “snap shut” lids on these individual 0.5mL tubes for PCR applications. X1000 PCR TUBE 0,5ML FLAT ASSORTED(VE=1000Stck.)
Invitrogen™ RNase-free Microfuge Tubes, 1.5mL
Autoclavable X500 RNASE-FREE MICROFUGE TUBES (1.5ML) STORE ATroom temperature