Protein Extraction and Purification

Thermo Scientific™ Pierce™ RIPA Buffer

Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent. RIPA BUFFER, 250 MLlysis and extraction buffer 250mL Store at 4C,

Invitrogen™ Total Exosome Isolation Reagent (from cell culture media)

Easily isolate exosomes and avoid time-consuming ultra-centrifugation Total Exosome Isolation (from Cell Culture Media),50 ml

Triton™ X-100 (Electrophoresis), Fisher BioReagents™

CAS: 9002-93-1 Molecular Formula: C16H26O2 Molecular Weight (g/mol): 250.382 InChI Key: JYCQQPHGFMYQCF-UHFFFAOYSA-N Synonym: Polyethylene Glycol p-tert-Octylphenyl Ether PubChem CID: 5590 500ML Triton X-100 for Electrophoresis

Thermo Scientific™ Pierce™ LAL Chromogenic Endotoxin Quantitation Kit

Accurately measure endotoxin levels (LPS, lipopolysaccharides) in protein, peptide or antibody samples using our Limulus Amebocyte Lysate (LAL) assay. PIERCE LAL CHROMOGENIC ENDOTOXIN QUANTITATION

Sodium Dodecyl Sulfate (SDS), White Powder, Electrophoresis, Fisher BioReagents™

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M Synonym: Sodium Lauryl Sulfate, SDS PubChem CID: 3423265 ChEBI: CHEBI:8984 5KG Sodium Dodecyl Sulfate (SDS), Electrophoresis(White Powder), (as Fatty Alcohol Sulfate):

GE Healthcare HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). HISTRAP HP 5ML

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Pierce Protease Inhibitor Mini Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments X30 Pierce Protease Inhibitor Mini Tablets

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktails

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors. HALT PROT&PHOSPH INHIB EDTA FR 5ML

Invitrogen™ MagnaRack™ Magnetic Separation Rack

Holds up to 24 microcentrifuge tubes MAGNARACK 24each

GE Healthcare Sera-Mag SpeedBeads™ Carboxyl Magnetic Beads, hydrophobic

Excellent for coupling oligos or proteins directly to the beads. Their “cauliflower-like” structure gives Sera-Mag beads high binding capacity. The carboxyl groups on these beads can be activated by standard EDC/EDAC chemistry to react with free amino groups, forming amide bonds. 1000mL SpeedBead magnetic carboxylate modified particles,DSMG-CM, 1 um, 5% solids

Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates. X5 HISPUR NI-NTA PURIFICATION KIT

GE Healthcare HisTrap™ FF Columns: 5 x 5mL

Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media. X5 HISTrap Fast flow 5mL

Invitrogen™ Magnetic Stand-96

Designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates and 0.2mL PCR plates without additional accessories MAGNETIC STAND-96 1 STANDFor the paramagnetic bead precipitation from

Invitrogen™ eBioscience™ RBC Lysis Buffer, 1x

Formulated for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic tissues such as spleen and human peripheral blood. eBioscience 1X RBC LysisBuffer 200 ml

Thermo Scientific™ Pierce™ NHS-Activated Magnetic Beads

Immobilize proteins or other ligands for magnetic separation affinity purification with these magnetic particles coated with amine-reactive NHS-ester groups. 1 ML NHS-ACTIVATED MAGNETIC BEADS 1ML STORE INfridge at 4C

GE Healthcare HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). X100 HisTrap(TM) FF crude, ready to use column,

Thermo Scientific™ Pierce™ Disposable Plastic Columns

Empty drip columns (0.5 to 5 mL) with matching frits and caps for packing beaded affinity or desalting resins to use in gravity-flow purification techniques. COLUMN PIERCE PLUS 2 EACH OF PN29920, PN29922,PN29924 and 1 of PN29923 (trial pack) disposable

Thermo Scientific™ Pierce™ Lysozyme

Degrade unwanted bacterial cell wall in protein or nucleic acid extracts 5 GR LYSOZYME 5G STORE IN FREEZER AT -20C

Thermo Scientific™ M-PER™ Mammalian Protein Extraction Reagent

Isolate total protein from cultured mammalian cells with this highly efficient lysis and extraction reagent. 1 LT MAMMALIAN PROTEIN EXTRACTION REAGENT M-PER1000mL

GE Healthcare Streptavidin Sepharose™ High Performance Medium

Used for convenient, fast, reliable purification of biotinylated compounds. Streptavidin Sepharose™ High Performance Medium is a physically and chemically stable medium. STREPTAVIDIN SEPHAROSE HP,5 ML

Thermo Scientific™ Halt™ Protease Inhibitor Cocktails

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors. 10 ML HALT(TM) PROTEASE INHIBITOR COCKTAIL, EDTA-Free 100x 10mL

EMD Millipore™ BugBuster™ Protein Extraction Reagent

Reagent for protein sample preparation 100ML BUGBUSTER PROTEIN EXTRACTION REAGENT, SIZE =

Thermo Scientific™ Pierce Protease Inhibitor Tablets

Thermo Scientific™ Pierce Protease Inhibitor Tablets are broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation for cell lysis and protein extraction experiments X20 Pierce Protease Inhibitor Tablets, EDTA-Free

Sodium Dodecyl Sulfate (SDS), Micropellets, Fisher BioReagents

CAS: 151-21-3 Molecular Formula: C12H25NaO4S Molecular Weight (g/mol): 288.378 InChI Key: DBMJMQXJHONAFJ-UHFFFAOYSA-M PubChem CID: 3423265 ChEBI: CHEBI:8984 5KG Sodium dodecyl sulfate (SDS) micropellets

Thermo Scientific™ Low Protein Binding Collection Tubes, 1.5 mL

Minimize protein sample loss during sample manipulation, especially when there are many processing steps, by using Thermo Scientific™ Pierce™ Low Protein Binding Microcentrifuge Tubes (1.5 mL). X2500 Pierce Low Protein Binding MicrocentrifugeTubes, 1.5mL, 10 x 1pk/250 tubes

GE Healthcare His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification. X10 HIS GRAVITRAP

Fisher Bioreagents™ Protease Inhibitor Cocktail III, Animal Free (For Mammalian Cells)

Supplied in 1mL DMSO. Fisher Bioreagents Protease Inhibitor Cocktail III, Animal Free (For Mammalian Cells) is a mixture of six protease inhibitors for use in applications that require animal free reagents. 1 ML Protease Inhibitor Cocktail III Animal Free

Thermo Scientific™ Pierce™ Protease Inhibitors

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds. LEUPEPTIN 50MG

GE Healthcare HiTrap™ Heparin HP Columns

Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. GE Healthcare Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification. X5 HITrap Heparin High performance 5mL

Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Kit

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit. 15ML NUCLEAR AND CYTOPLASMIC EXTRACTION REAGENT,

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