Antibotics, Buffers and Supplements

Gibco™ Trypsin-EDTA (0.25%), Phenol red

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas TRYPSIN 0.25% EDTA

Gibco™ Trypsin-EDTA (0.05%), Phenol red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN .05% EDTA

Gibco™ TrypLE Express Enzyme (1X), No Phenol Red

Unlike porcine trypsin, free of animal-derived components TRYPLE EXPRESS

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test TRYPAN BLUE STAIN

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses 100ML Cell Recovery Solution

Gibco™ Trypsin-EDTA (0.5%), No Phenol Red

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas 100ML 0.5% Trypsin-EDTA (10x), no phenol redin plastic container, E-Z hold Store at -20C

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells INSULIN-TRANS-SEL-G, 100X

Gibco™ Trypsin (2.5%), 10X Concentration

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas TRYPSIN, 2.5%, 10X

Gibco™ HEPES (1M)

A zwitterionic organic chemical buffering agent commonly used in cell culture media HEPES (1M) 100ML

Gibco™ Sodium Pyruvate (100mM)

Growth supplement 100ML Sodium Pyruvate

Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability 100 ML MEM NON-ESSENTIAL AMINO ACIDS SOLUTION10mM (100X), liquid 100mL RRR - pack at ambient

Gibco™ ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow ACK LYSING BUFFERACK Lysing Buffer is suitable for use in the

Corning™ Trypan Blue

Can be used for variety cell counting methods requiring information on cell count and viability. 100 mL Trypan Blue Solution in PBS 0.4% (w/v)

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells. 5ML MatrigelhESC-Qualified Matrix, LDEV-Free

Gibco™ L-Glutamine (200mM)

Ready-to-use stock solution that acts as a cell culture supplement 20ML L GLUTAMINE, 100X

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression. 5ML Matrigel Growth Factor Reduced (GFR) Basement

Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis. 10MG Cell-Tak Cell and Tissue Adhesive

Gibco™ 2-Mercaptoethanol (55mM)

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals 2 MERCAPTOETHANOL 1000X

Gibco™ TrypLE Express Enzyme (1X), Phenol Red

Animal origin-free, recombinant enzyme X20 Tryple express kit with phenol red store at

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.302 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: C1CN(CCN1CCO)CCS(=O)(=O)O 5KG HEPES (Fine White Crystals) for Molecular Biology,

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression. 50ML Matrigel Basement Membrane Matrix, LDEV-Free

Gibco™ Low Serum Growth Supplement (LSGS)

Low Serum Growth Supplement (LSGS) LSGS 10MLdermal fibroblasts + endothel cells

Gibco™ Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix

Geltrex™ hESC-Qualified, Ready-To-Use, Reduced Growth Factor Basement Membrane Matrix GELTREX HESC QUAL READY TO USE

Gibco™ Pluronic F-68 Non-ionic Surfactant (100X)

A non-ionic surfactant used to control shear forces in suspension cultures PLURONIC

Corning™ DMSO (Dimethyl Sulfoxide)

For cryopreservation of cells along with glycerol, to prevent damage to cell membrane during freezing 250 mL DMSO (Dimethyl Sulfoxide)

Gibco™ Human Keratinocyte Growth Supplement (HKGS)

Human Keratinocyte Growth Supplement (HKGS) HKGS 5MLA sterile, concentrated (100X) solution intended

Gibco™ Bacto™ Tryptone

Enzymatic digest of casein. Gibco™ Bacto™ Dehydrated Culture Media Additive: Tryptone is part of a large selection of dehydrated media. 500GR TRYPTONE

Gibco™ Bacto™ Peptone

Enzymatic digest of protein 10KG PEPTONE

Corning™ ITS Premix Universal Culture Supplement

Stimulates proliferation of a variety of cells under serum-reduced conditions. Corning™ ITS Premix Universal Culture Supplement contains insulin, human transferrin and selenous acid, the three most universally esential components of defined culture media. 5ML ITS Premix Universal Culture Supplement

MES (Fine White Crystals), Fisher BioReagents

100GR MES monohydrate (Fine White Crystals),

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