Western Blotting Reagents, Membranes, Supplies

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity.  Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

An enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis

Invitrogen™ iBlot™ Transfer Stack, nitrocellulose, mini

iBlot Transfer Stacks are used to transfer proteins

Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, mini

Used to transfer proteins with the iBlot 2 Gel Transfer Device

Invitrogen™ Amplex™ UltraRed Reagent

Improves upon performance of Amplex Red reagent

Invitrogen™ XCell SureLock™ Mini-Cell and XCell II™ Blot Module

Quickly and easily run and blot Novex minigels

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) HRP substrate with very stable light output for femtogram-level detection with CCD-based imagers.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls

Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Invitrogen™ Incubation Tray, 10 x 14 cm

Enables convenient and even staining of E-PAGE™ blots

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Hyperfilm™ ECL

Use with all blue and green light chemiluminescent systems. Cytiva Amersham™ Hyperfilm™ ECL is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer.

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Hybond™ P 0.45μm PVDF Membrane

Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.

Invitrogen™ iBlot™ Transfer Stack, nitrocellulose, regular size

iBlot Transfer Stacks are used to transfer proteins

Invitrogen™ iBlot™ 2 Transfer Stacks, nitrocellulose, regular size

Used to transfer proteins with the iBlot 2 Gel Transfer Device

Cytiva (Formerly GE Healthcare Life Sciences) Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls

Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Invitrogen™ iBlot™ 2 Starter Kit

The iBlot 2 Starter kit includes the iBlot 2 Gel Transfer Device as well as iBlot 2 Transfer Stacks (nitrocellulose) - everything needed to perform a rapid dry transfer of proteins from a gel to a membrane.

Merck Millipore Luminata Forte Western HRP substrate, 100mL

Premixed, ready to use chemiluminescent HRP detection reagent

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Invitrogen™ PVDF/Filter Paper Sandwich, 0.2 µm, 8.3 x 7.3 cm

Pre-cut and pre-assembled—ready for protein blotting/transfer

Hoefer™ TE22 Mini Tank Blotting Unit

Safe, economical unit requires minimal current and only about 1L of buffer

Invitrogen™ iBind™ Flex Cards

Card for use with iBind™ Flex Western Blot Device

Invitrogen™ iBlot™ 2 Transfer Stacks, PVDF, mini

Consumable stacks with integrated PVDF transfer membranes for dry blotting of proteins

Thermo Scientific™ Western Blotting Filter Paper, Extra Thick, 8 cm x 13.5 cm

Pre-cut cotton sheets for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels to PVDF, nitrocellulose, or other membranes.

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