Les milieux de culture cellulaire
Gibco™ StemPro™-34 SFM (1X)
StemPro™-34 SFM (1X)
Gibco™ Expi293™ Expression Medium
Expi293™ Expression Medium
Gibco™ Cell Dissociation Buffer, enzyme-free, PBS
Animal origin-free
Gibco™ Ham's F-12 Nutrient Mix
Designed for serum-free, single-cell plating of Chinese hamster ovary (CHO) cells
Gibco™ Versene Solution
An EDTA solution for use as a gentle non-enzymatic cell dissociation reagent
Gibco™ AmnioMAX™-II Complete Medium
Fully-supplemented medium developed for short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures
Gibco™ Advanced DMEM/F-12
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ Advanced DMEMF/12 is used in cell culture processing applications.
HyClone™ DMEM/F12 1:1 Media
Used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. HyClone™ DMEM/F12 1:1 Media supports cell culture growth and biomanufacturing processes and is manufactured using ISO 9001:2000-certified processes.
HyClone™ SFM4Insect™ Media
Support growth of many key insect cell lines and production of a variety of recombinant proteins. HyClone™ SFM4Insect™ Media is free of animal-derived components (ADCF).
HyClone™ McCoy's 5A Media
Support cell culture growth and biomanufacturing processes with HyClone™ McCoy's 5A Media, manufactured using ISO 9001-certified processes.
Gibco™ DMEM, high glucose, HEPES, no phenol red
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, HEPES, No Phenol Red is used in cell culture processing applications.
Lonza™ BioWhittaker Insect-XPRESS Insect Cell Culture Media (1X) with L-Glutamine
Designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21). BioWhittaker™ Insect-XPRESS™ Insect Cell Culture Media (1X) with L-Glutamine supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS).
Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, GlutaMAX™ Supplement, Pyruvate is used in human ex vivo tissue and cell culture processing applications.
Gibco™ Ham's F-12K (Kaighn's) Medium
A modification of Ham's F-12 Nutrient Mixture
Gibco™ Glucose Solution
Contains D-glucose at concentration of 200 g/L and is used as serum-free medium supplement suitable for cell culture
Gibco™ Opti-MEM™ I Reduced Serum Medium, no phenol red
Improved Minimal Essential Medium that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in cell growth rate or morphology
Gibco™ Essential 8™ Medium
Essential 8™ Medium
Gibco™ DMEM/F-12, GlutaMAX™ supplement
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEMF/12, GlutaMAX™ Supplement is used in cell culture processing applications.
Gibco™ DMEM/F-12, no phenol red
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEMF/12, No Phenol Red is used in cell culture processing applications.
Gibco™ RPMI, no methionine
Developed to culture human leukemic cells in suspension and as monolayer
Gibco™ RPMI 1640 Medium, no folic acid
Originally developed to culture human leukemic cells in suspension and as a monolayer
Gibco™ IMDM
Iscove's Modified Dulbecco's Medium is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
Gibco™ Recovery™ Cell Culture Freezing Medium
Complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3
Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES
Originally developed to culture human leukemic cells in suspension and as a monolayer
Gibco™ DMEM, high glucose, pyruvate
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, Pyruvate is used in cell culture processing applications.
Gibco™ IMDM, no phenol red
Suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells
Gibco™ Opti-MEM™ I Reduced Serum Medium
Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.
Gibco™ DMEM, high glucose, no glutamine
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, No Glutamine is used in cell culture processing applications.
Gibco™ DMEM, powder, high glucose, pyruvate
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, Powder, High Glucose, Pyruvate, No Sodium Bicarbonate Buffer is used in cell culture processing applications.
Gibco™ MEM α, nucleosides, no ascorbic acid
Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells
Les milieux de culture cellulaire soutiennent la croissance et la conservation de diverses lignées cellulaires de mammifères et autres in vitro, sous forme de cultures adhérentes ou en suspension. La culture cellulaire garantit la praticité, le contrôle, la constance et la reproductibilité des résultats, en particulier lors de l’utilisation de cellules clonales.
La culture cellulaire comporte des applications multiples dans le domaine de la biologie cellulaire et moléculaire:
- Étude de la physiologie cellulaire normale et de la biochimie
- Fabrication de vaccins, de protéines thérapeutiques et d’anticorps monoclonaux à l’aide de bioprocédés industriels / d’un bioréacteur grâce à des systèmes d’expression des protéines recombinants et à haut rendement
- Analyse et développement de produits pharmaceutiques, y compris le métabolisme des médicaments et la toxicologie
- Autres applications émergentes dans le domaine de la culture cellulaire, de l’immunothérapie et de la thérapie cellulaire
Les milieux de culture disponibles dans le commerce sont soumis à des tests et à des contrôles de fabrication extrêmement stricts. Ils sont regroupés en différentes catégories:
- Milieux classiques de culture de cellules de mammifères (DMEM, MEM, RPMI 1640, de McCoy, de Ham, BME et EBSS) contiennent tous les nutriments dont les cellules ont besoin pour croître, tels que des acides aminés, des minéraux, des vitamines et du glucose
- Les suppléments, additifs et réactifs pour milieux individuels, y compris les facteurs de croissance (par exemple le pyruvate), les antibiotiques, les hormones, les milieux de cryoconservation, les réactifs de dissociation cellulaire et les sérums d’origine animale (par exemple le sérum du veau fœtal [SVF]), qui contiennent un mélange naturel de nutriments prêt à l’emploi
- Les milieux spéciaux, y compris les milieux pour cellules souches, les milieux de transfection et les additifs sans sérum ou exempts de composés d’origine animale (ADCF)
- Les tampons accessoires et réactifs, y compris les solutions salines équilibrées (tampons au phosphate salin PBS, HBSS de Hank, EBBS de Earle), les tampons au bicarbonate de sodium et HEPES, le rouge de phénol, l’eau pour culture cellulaire et les microsupports pour cellules adhérentes
Les milieux de culture cellulaire sont normalement fournis sous forme de liquides, de concentrés, de poudres ou d’autres solides prêts à l’emploi et préstérilisés, en flacons ou en sachets étanches à usage unique pour une intégration aux systèmes de récolte de culture cellulaire compatibles.
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories:
Commercially available cell culture media are subject to very stringent testing and manufacturing controls. They fall into several categories: