PepMap Columns

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The standard for Peptide Separations in Proteomics

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Thermo Scientific™Acclaim™ PepMap™ and Acclaim™ PepMap™ RSLC columns are specially designed for high-resolution analyses of tryptic, natural, and synthetic peptides. The columns are often applied for LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology. Due to their high loading capacity, the columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples.

PepMap columns are available in separation (analytical) and trap formats.

Key features & benefits

  • High resolution protein identification for biomarker discovery, verification or any other analysis
  • Highest sensitivity in LC-MS
  • Higher throughput due to higher loading pressure
  • Thermo Scientific™ nanoViper™ fingertight connections for easy column installation

PepMap columns employ nanoViper zero-dead-volume (ZDV) fingertight connections that eliminate the assembly of PEEK sleeve connections making it far easier to achieve state-of-the-art nanospray performance and are capable of withstanding pressures up to 1200 bar.

 

Separation columns
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Separation columns come in diameters for nano (50 and 75 μm), capillary (300 μm) and micro (1000 μm) LC. Different lengths are available from 5cm for high throughput with short micro columns to 75 cm long nano columns for high peak capacity and sensitivity.

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Trap columns
nanotrap

The Acclaim® PepMap100 Nano-Trap columns are available in 100 μm and 200 μm i.d. fused silica, and are packed with 5 μm particle size and 300 Å pore size.

The nano trap design features a single capillary with the trap column at the end and is ideal for high resolution separation of relatively clean samples or samples with hydrophilic peptides.

The cartridge based trap columns have a wider ID and are ideal for large sample amounts or when sample quality demands more robustness.

    Catalog No. Description Price Stock Quantity  
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11352013 Details
X2 HPLC column Thermo Scientific Dionex Acclaim(R) 628.13€
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TRUSTED_SUSTAINABILITY
  
Product Specifications
Carbon Load 15%
Length (Metric) 150 mm
Quantity Pack of 2
Pore Size 100 Å
Type Trap Column
Particle Size 3 μm
For Use With UltiMate 3000 RSLC Nano System
Material PEEK Shielded Fused Silica
Temperature 60°C
USP Type L1
Column Type Reversed Phase
Packing Material Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric) 0.075 mm
Bed Length 20 mm
Product Line nanoLC
Stationary Phase C18
Column Format Trap Column, NanoViper
Max. Pressure 7250 psi (500 bar)
Surface Area 450 m²/g
Phase Reversed Phase
Endcapped Yes
pH 2 to 8
11312263 Details
X2 HPLC column Thermo Scientific Dionex Acclaim(R) 499.00€
Expédition estimée: 26-05-2025
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TRUSTED_SUSTAINABILITY
  
Product Specifications
Carbon Load 15%
Length (Metric) 150 mm
Pore Size 100 Å
Type Trap Column
Particle Size 5 μm
For Use With UltiMate 3000 RSLC Nano System
Temperature 60°C
USP Type L1
Column Type Peptide Analysis
Packing Material Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric) 0.1 mm
Bed Length 20 mm
Stationary Phase C18
Column Format Trap Column, NanoViper
Max. Pressure 7250 psi (500 bar)
Surface Area 450 m²/g
Phase Reversed Phase
Endcapped Yes
pH 2 to 8
Trap cartridges
MCA3

Acclaim® PepMap™ μ-Precolumns are very short microcolumns–available in 5 and 15 mm lengths–consisting of a cartridge holder and a set of disposable cartridges. μ-Precolumns are used for sample pre-concentration, sample cleanup, or in various column-switching techniques.

    Catalog No. Description Price Stock Quantity  
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