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Thermo Scientific™ Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
Marque: Thermo Scientific™ F519L
21.56 EUR valable jusqu'au 2024-12-20
Utilisez le code promo "21651" pour bénéficier de cette offre.
Description
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
Tampon GC pour ADN polymérases Phusion Développé pour les modèles riches en CGComprend :
Tampon Phusion GC 5x, solution de MgCl2 de 50 mM, DMSO à 100 %.
Spécification
Le pack de tampons Phusion GC comprend : • Tampon Phusion GC 5X (tampon 1X fournissant 1,5 mM de MgCl2) • 50 mM de MgCl2 • DMSO Conserver à -20°C. |
|
ADN polymérase Phusion haute fidélité | |
Wet or Dry Ice | |
ADN | |
Colorless |
Boîte de tampons Phusion™ GC | |
GC Buffer Pack | |
PCR standard, High-fidelity PCR | |
4 x 1,5 mL |
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.