Invitrogen™ Kit ELISA pour IL-20 de souris

Description

The Mouse Interleukin-20 (Ms IL-20) ELISA quantitates Ms IL-20 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-20. Principle of the method The Mouse IL-20 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Les cytokines qui émettent des signaux par les récepteurs de classe II forment une famille distincte qui comprend les interférons et lʼinterleukine 10 (IL-10). Lʼidentification récente de plusieurs homologues IL-10 a défini une sous-famille de cytokine incluant AK155, IL-19, IL-20, IL-22 et IL-24. L’IL-20 appartient à la famille IL-10 et joue un rôle dans lʼinflammation de la peau et le développement des cellules hématopoïétiques. Peu dʼinformation a été obtenue sur ses autres fonctions biologiques et ses implications cliniques. Ces cytokines affichent des activités pro-inflammatoires dans différents tissus, y compris la peau.