Dissociation Reagents

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Gibco™ GlutaMAX™ Supplement

An alternative to L-glutamine, with increased stability that improves cell health

Gibco™ Trypan Blue Solution, 0.4%

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ MEM Non-Essential Amino Acids Solution (100X)

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ ACK Lysing Buffer

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Gibco™ L-Glutamine (200 mM)

Ready-to-use stock solution that acts as a cell culture supplement

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ Sodium Bicarbonate 7.5% solution

Commonly used for maintaining the pH of cell culture medium in the presence of 4 to 10% carbon dioxide

Gibco™ Pluronic™ F-68 Non-ionic Surfactant (100X)

A non-ionic surfactant used to control shear forces in suspension cultures

Gibco™ Phytohemagglutinin, M form (PHA-M)

This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1 to 2% v/v

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Corning™ Fibronectin, Human

Promotes cellular attachment, spreading, and proliferation on tissue-culture surfaces. Corning™ Fibronectin, Human can be used as an additive to serum-free culture media.

Gibco™ Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.

Invitrogen™ Tryptose Phosphate Broth

Cell culture supplement for BHK-21 cells used for vaccine production

Gibco™ 2-Mercaptoethanol

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Corning™ Matrigel™ Membrane Matrix HC

High concentration (HC) formulation is ideal for applications in which a high protein concentration augments the growth of tumors; allows a Matrigel basement membrane matrix plug to maintain its integrity after subcutaneous injection into mice

Gibco™ HT Supplement (100X)

HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.

Gibco™ KaryoMAX™ Giemsa Stain Solution

For G-banding of chromosomes for cytogenetic analysis

Gibco™ Autolyzed Yeast Extract (15% solution)

A concentrated extract of soluble amino acids, carbohydrates, vitamins and minerals

Corning™ PuraMatrix™ Peptide Hydrogel

Highly defined synthetic peptide assembles into fibrous 3D structure and promotes cell attachment

Gibco™ HAT Supplement (50X)

HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells

Gibco™ Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X)

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Corning™ Nu-SerumTM Growth Medium Supplement

Provides a low-serum alternative to newborn calf, fetal bovine, and other sera used for cell culture

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