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Invitrogen™ Thyroxine (T4) Competitive ELISA Kit

Competitive ELISA

610.00€ - 4635.00€

Spécification

Sensibilité du dosage 0,29 ng
Description Kit ELISA compétitif pour thyroxine (T4)
Conjugué HRP
Méthode de détection Fluorescence
Type de produit Kit ELISA
Consulter d'autres spécifications

Produits 2
Code produit Marque Suffisant pour Prix Quantité et disponibilité  
Code produit Marque Suffisant pour Prix Quantité et disponibilité  
15845863
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Invitrogen™
EIAT4C
96 dosages
610.00€
96 pièces
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17865217
Afficher les documents Détails de la promotion
Invitrogen™
EIAT4CX10
10 x 96 dosages
4635.00€
10 pièces
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Description

Description

The Thyroxine (T4) ELISA quantitates Thyroxine (T4) in serum, plasma, urine, dried fecal extracts, or cell culture medium. The assay will exclusively recognize both natural and recombinant Thyroxine (T4). Principle of the method The Thyroxine (T4) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Thyroxine is the main hormone produced by the thyroid gland. The thyroid hormones, triiodothyronine (T3) and thyroxine (T4), are tyrosine-based hormones produced by the thyroid gland that are primarily responsible for regulation of metabolism. Iodine is necessary for the production of T3 and T4. The major form of thyroid hormone in the blood is thyroxine, which has a longer half-life than T3. T4 is converted to the active T3 within cells by deiodinases (5’-iodinase). These are further processed by decarboxylation and deiodination to produce iodothyronamine and thyronamine. Thyroid hormone production is regulated via pituitary thyrotropin (TSH) modulation of thyroxine prohormone secretion by the thyroid gland and regulation of active triiodothyronine production. Thyroxine is identical across all species.
Spécification

Spécification

0,29 ng
HRP
Kit ELISA
Humaine
Lecteur de microplaques colorimétriques
3%
RUO
-20°C
1 heures 20 min
Kit ELISA compétitif pour thyroxine (T4)
Fluorescence
Extrait fécal, Sérum, Surnageant, Surnageant, Urine
ELISA, dosages de protéines, tests d’activité, biologie des protéines, biologie de la reproduction, inflammation, stress oxydatif, métabolisme, signalisation cellulaire, épigénétique, hématologie, dommages à l’ADN, biologie du cancer, immunologie, neurosciences, neurobiologie, détection d’hormones stéroïdes
7%
HRP
Métabolisme
Produits chimiques
1 h 30 min
Vidéos
FDS
Documents

Documents

Product Certifications