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Thermo Scientific™ 1-Step CHO High-Yield IVT Kit
Express of functional proteins with much greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems using the Thermo Scientific™ 1-Step CHO High-Yield In Vitro Translation (IVT) Kit.
Marke: Thermo Scientific™ 88893
Beschreibung
The Thermo Scientific 1-Step CHO High-Yield In Vitro Translation (IVT) Kit enables the expression of functional proteins with up to 750 times greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems that only yield up to 10μg protein/mL reaction.
- High expression - up to 750μg/mL of expressed protein
- Reproducible results - low variability between experiments
- Fast - express high levels of protein with 6 hours to overnight incubation
- Easier storage - allows you to store lyophilized CHO cell lysate at -20°C
- Functional - obtain functionally active proteins, including those containing disulfide bonds
- Easy-to-use - three reaction components together carry out transcription and translation in one step
- Great for labeling - amenable to incorporation of labeled and unnatural amino acids
- Vector compatibility - uses existing pT7CFE1 vectors for protein expression
Includes:
Lyophilized cell lysate, reaction mix, accessory proteins, 4X dialysis buffer, control DNA, cloning vector, dialysis devices, and centrifuge tubes.
Requires:
30°C incubator or water bath (shaker incubator increases expression by 30 to 50%)
Applications:
- Production of large amounts of recombinant protein for structural analysis
- Generation of active proteins for downstream applications such as protein interaction studies
- Rapid large-scale expression and characterization of mutant proteins
- Production of viral particles
- Incorporation of unnatural amino acids
- Production of cytotoxic proteins
Spezifikation
Multi-component kit | |
750 Times greater yield per mL | |
2 Reactions of 100μL each | |
Store in ultra-cold freezer (-68°C to -85°C) |
1-Step™ CHO High-Yield IVT Kit, 2 x 100μL, Cell-Free Expression, Sufficient for 2 Reactions Of 100μL Each, –68 to –85°C Store In Ultra-Cold Freezer, Production of Large Amounts of Recombinant Protein for Structural Analysis | |
1-step CHO High-Yield IVT Kit | |
2 x 100 μl | |
Protein biology, protein expression, cell-free (In-vitro) expression, In-vitro translation, production of large amounts of recombinant protein for structural analysis, generation of active proteins for downstream applications such as protein interaction s |
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