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IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity FPLC column
Strep-Tactin™ 4Flow™ high capacity FPLC column to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
Marque: IBA Lifesciences 2-1258-001
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Description
- Strep-Tactin™ 4Flow™ high capacity FPLC columns are designed for purifying Strep-tag™II and Twin-Strep-tag™ proteins via affinity chromatography in automated protein purification with HPLC/FPLC devices such as Äkta™ systems (Cytiva).
- Recommended for all protein classes due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity
- 4% crosslinked agarose beads coupled to Strep-Tactin™ for straightforward purification of proteins with Strep-tag™II or a Twin-Strep-tag™
- Multiple regeneration cycles possible without loss of binding capacity
- Ideal alternative for proteins that are difficult to elute from Strep-Tactin™XT 4Flow™ resins
- Biotinylated proteins also bind to Strep-Tactin™ 4Flow™ high capacity and need to be blocked using BioLock
Spécification
| 20 mg/mL resin | |
| 4% agarose, crosslinked | |
| Resin Slurry | |
| Tetrameric protein | |
| Column | |
| Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin | |
| FPLC cloumn |
| 5 mL | |
| 50 to 150 μm | |
| 5 mL | |
| Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin | |
| Affinity chromatography equipment | |
| Agarose | |
| Pre-packed gravity flow column |