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Invitrogen™ Rat TNF alpha ELISA Kits
Measure levels of rat tumor necrosis factor alpha (TNFa) levels in rat serum, plasma and culture supernatant samples using 96-well plates.
Marque: Invitrogen™ ER3TNFA
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Description
Assay TNFα (rat tumor necrosis factor alpha) levels in rat serum, plasma (EDTA, heparin) and culture supernatant samples with our Thermo Scientific Rat TNF alpha ELISA Kits.
These rat TNF alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of tumor necrosis factor alpha (TNF-alpha, TNFα) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant rat TNFα (rat tumor necrosis factor alpha)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 31 to 2500pg/mL
Sensitivity: < 15pg/mL
Precision: Intra-assay CV < 10%; inter-assay CV < 10%
Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%)
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Spécification
| 31 to 2500pg/mL | |
| Rat TNF alpha ELISA Kit | |
| Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum | |
| 96 puits de microplaques | |
| Absorbance à 450 nm moins absorbance à 550 nm | |
| 1 Plate Kit | |
| Natural and recombinant rat TNFa (rat tumor necrosis factor alpha) |
| <15pg/mL | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| No significant cross-reaction with human TNFα, human TNFβ or porcine TNFα. Mouse TNFα cross-reacts weakly (< 3%) | |
| Standard Diluent, 25mL, TMB Substrate Solution, 13mL, Stop Solution, 13mL, Pre-Treatment Buffer, 6mL, Lyophilized Recombinant Rat TNF alpha Standard, 2 vials, Biotinylated Antibody Reagent, 6mL, Streptavidin-HRP Reagent, 14mL, Anti-Rat TNF alpha Precoated 96-well Strip Plate, 1 ea, 30X Wash Buffer, 50mL | |
| Intra-assay CV <10%; inter-assay CV <10% | |
| 50 μl | |
| 3 hr. |