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Thermo Scientific™ Kit de transfert Western rapide Pierce™ SuperSignal West Femto
Pour la détection de niveau de femtogramme moyen à faible et le développement de transfert Western moins d’1 heure après le transfert du gel.
Marque: Thermo Scientific™ 35086
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Description
Le substrat SuperSignal West Femto du kit pour transfert Western rapide Thermo Scientific™ Pierce contient des réactifs et un protocole optimisés qui permettent de détecter les niveaux moyens à faibles de femtogramme et réduit à moins d’1 heure le développement de transfert Western typique après le transfert de gel.
The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrates sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.Highlights:
Fast – low femtogram levels of detection in about 1 hour
Convenient – no expensive hardware or vacuum required; no clogging issues
Simple – optimized protocol makes Western blot analysis easier than ever
Economical – no additional consumables or extra equipment to purchase
Stable – all kit components are stable for one year stored at 4°C
Easy – complete kits contain all components needed to block, probe and develop a blot with any standard mouse or rabbit primary antibody
Compatible with:
Pierce Membranes and Filter Paper; CL-Xposure™ Film for Western blotting; Pierce Western Blot Signal Enhancer; Restore Plus™ Western Blot Stripping Buffer
Spécification
| Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate, Rabbit Trial Size | |
| 80 mL Kit | |
| 2 mini blots probed with rabbit primary antibodies |
| Chemiluminescence | |
| HRP | |
| SuperSignal West Femto Luminol/Enhancer Solution, 10mL, SuperSignal West Stable Peroxide Solution, 10mL, Fast Western Rabbit Optimized HRP Reagent, Femto, 2mL, Fast Western Antibody Diluent, 40mL, Fast Western 10X Wash Buffer, 20mL |
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