Invitrogen™ Kit ELISA pour TIMP2 humaine

Description

The Human Tissue Inhibitor of MetalloProteinases 2 (TIMP-2) ELISA quantitates Hu TIMP-2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TIMP-2. Principle of the method The Human TIMP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TIMP-1 et TIMP-2 sont des enzymes inhibitoires de la famille des métalloprotéinases matricielles que l’on pense avoir une grande importance dans la maintenance de l’intégrité du tissu conjonctif. TIMP-2 fait preuve de la plus haute affinité de liaison avec les deux formes, latente (pro) et active, de la collagénase de type IV de 72 kDa (aussi connue sous les appellations de MMP-2 ou gélatinase A). Elle a aussi une affinité pour la forme active de la collagénase de type IV de 92 kDa (aussi connue sous l’appellation de MMP-9 ou gélatinase B). TIMP-2 inhibe l’invasivité protéolytique des cellules tumorales et des cellules trophoblastiques placentaires normales.