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Gibco™ MEM, high glucose, high sodium bicarbonate
Utilisé avec diverses cellules de mammifères en suspension et adhérentes
Marque: Gibco™ A1451801
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Description
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using our media selector tool.
This MEM is modified as follows:
With: Phenol Red, D-glucose (6 g/L), Sodium Bicarbonate (3.7 g/L)
Without: L-glutamine, HEPES
Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Certifications
ISO 13485
Spécification
| Conditions de stockage : 2 à 8 deg;C. À l’abri de la lumière Conditions d’expédition : température ambiante Durée de conservation : 12 mois à compter de la date de fabrication |
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| Astrocytes de rat primaires | |
| 1 X | |
| MEM (milieu essentiel minimal) | |
| Stérilisation par filtration | |
| Sans glutamine, Sans HEPES, Sans pyruvate de sodium | |
| Emballage durable | |
| Gibco™ | |
| Usage exclusivement réservé à la recherche. Non utilisé à des fins diagnostiques ou thérapeutiques humaines ou animales. |
| HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7 et fibroblastes | |
| Aucun composant d’origine animale | |
| Liquide | |
| Supplémentation en sérum standard | |
| Haute teneur en glucose, Rouge de phénol, Bicarbonate de sodium | |
| Culture de cellules de mammifères | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL | |
| Température ambiante |