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Invitrogen™ Cortisone Competitive ELISA Kit
Competitive ELISA
Marque: Invitrogen™ EIACORX10
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.
Description
The Cortisone ELISA quantitates Cortisone in serum,saliva,urine,plasm,or dried fecal extracts.
Principle of the method
The Cortisone Competitive ELISA research-use-only kit is designed to quantitatively measure cortisone in extracted dried fecal samples,urine,saliva,plasma,and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two-hour incubation the plate is washed and TMB substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce a colored product. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Cortisone (Kendall’s Compound E) is a glucocorticoid which is the product of cortisol (Compound F) deactivation by 11-beta-HSD2 (hydroxysteroid dehydrogenase), prohibiting receptor activation. The balance between cortisone and cortisol glucocorticoids helps to regulate the anti-inflammatory response. Cortisone is identical across all species.Spécification
97,66-100,000 pg/ml | |
Kit ELISA compétitif pour cortisone | |
HRP | |
Kit ELISA | |
10 x 96 dosages | |
ELISA, dosages de protéines, tests d’activité, biologie des protéines, biologie de la reproduction, inflammation, stress oxydatif, métabolisme, signalisation cellulaire, épigénétique, hématologie, dommages à l’ADN, biologie du cancer, immunologie, neurosciences, neurobiologie, détection d’hormones stéroïdes | |
11,9% | |
Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection conjugué au HRP, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives | |
RUO | |
Extrait fécal, 50 μl ; plasma, 1 μl ; salive, 10 μl ; sérum, 1 μl ; urine, 10 μl | |
Produits chimiques | |
2 h 30 min |
29 pg/ml | |
Dosage multiplex sur la plateforme Luminex | |
Fluorescent | |
Extrait fécal, Salive, Sérum, Sérum, Urine | |
Humaine | |
Lecteur de microplaques colorimétriques | |
8,6% | |
HRP | |
Inflammation | |
-20°C | |
1 h 20 min |