HLA-ABC Monoclonal Antibody (W6/32), Invitrogen™

Description

MA1-70111 detects HLA-ABC in human samples. MA1-70111 has been successfully used in flow cytometry, functional assay, and immunoprecipitation procedures. The MA1-70111 immunogen is purified membrane of human tonsil cells. Store at 4°C short term. For extended storage aliquot and store at -20°C. Avoid Freeze/Thaw Cycles.

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.