Goat IgG F(ab')2 Isotype Control, PE, Invitrogen™

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Goat IgG, also known as goat immunoglobulin G, is an antibody that is produced by the immune system of goats in response to the presence of antigens. IgG is a type of immunoglobulin that plays a role in the recognition and neutralization of pathogens, such as bacteria and viruses. It binds to specific antigens and triggers various immune responses, including phagocytosis and complement activation. Goat IgG can be used in research to study the immune response to pathogens, as well as to develop diagnostic tests and therapeutic interventions for infectious diseases.