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MTCO1 Monoclonal Antibody (1D6E1A8), Invitrogen™
Mouse Monoclonal Antibody
Marque: Invitrogen 459600
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Description
For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal. Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio. In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa). In mouse liver lysate a specific band below 37 kDa was detected. Recommended positive controls: ICC/IF - Pig retinal pigment epithelial cells, rat cerebellum primary cells IHC (P) - Human skeletal muscle and colon tissue, rat pancreas tissue, pig smooth muscle tissue WB - Mouse, human, bovine and rat heart mitochondria lysate Flow - HEK-293 cells Recommended long term storage at -20°C.
MTCO1 Monoclonal antibody specifically detects MTCO1 in Human, Mouse, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot.
Spécification
| MTCO1 | |
| Monoclonal | |
| 1 mg/mL | |
| 59% PBS with 0.05% BSA, 40% glycerol and 0.01% sodium azide | |
| P05503, P00395, Q36347, O79876, P00397, Q9MIY8 | |
| COX1, MT-CO1 | |
| Human Mitochondrial Complex IV subunit I | |
| 100 μg | |
| Primary | |
| Human, Mouse, Rat | |
| Antibody | |
| IgG2a κ |
| Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot | |
| 1D6E1A8 | |
| Unconjugated | |
| MT-CO1 | |
| Co1; coi; cox1; coxi; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1; cytochrome c oxidase subunit I; mitochondrially encoded cytochrome c oxidase I; mtco1; mt-co1 | |
| Mouse | |
| Protein A | |
| RUO | |
| 140539, 17708, 26195, 4512, 1485858, 808503 | |
| 4° C, do not freeze | |
| Liquid |